Fujimura Takashi, Hori Ayane, Torii Hideto, Kishida Shinsuke, Matsuura Yoshinori, Kawamoto Seiji
1Hiroshima Research Center for Healthy Aging (HiHA), Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama Higashi-Hiroshima, Hiroshima, 739-8530 Japan.
Manda Fermentation Co., Ltd, 5800-95 Innoshima, Shigei, Onomichi, Hiroshima, 722-2192 Japan.
World Allergy Organ J. 2018 Dec 4;11(1):31. doi: 10.1186/s40413-018-0213-4. eCollection 2018.
Japanese cedar pollinosis (JCP) is one of the most prevalent allergies in Japan. Within the past few decades, many food factors have been demonstrated to suppress symptoms of pollinosis and mast cell degranulation directly or indirectly. Herein, we conducted a study to clarify the anti-allergic potency of a fermented plant product (FPP) in JCP model mice.
Mice were administered FPP, 10-fold-diluted FPP, or saline every day for 40 days by oral gavage and sensitized with major Japanese cedar pollen allergens (SBP). The numbers of sneezes were counted for 5 minutes after SBP nasal challenge. We analyzed the SBP-specific immunoglobulin titers, serum concentration of mast cell protease 1, and cytokine production from splenocytes stimulated with SBP.
The numbers of sneezes by the mice administered FPP were significantly suppressed compared to those administered saline. The 10-fold-diluted FPP also suppressed the number of sneezes compared to saline, although not significantly. Serum level of mast cell protease 1 tended to be suppressed in FPP-consumed mice compared to those in saline-treated mice. The SBP-specific immunoglobulin titers and cytokine production were comparable among the groups.
Our results suggest that FPP intake could attenuate JCP symptoms without change of systemic immune responses.
日本雪松花粉症（JCP）是日本最常见的过敏症之一。在过去几十年中，许多食物因素已被证明可直接或间接抑制花粉症症状和肥大细胞脱颗粒。在此，我们进行了一项研究，以阐明发酵植物产品（FPP）对JCP模型小鼠的抗过敏效力。
通过口服灌胃，每天给小鼠施用FPP、稀释10倍的FPP或生理盐水，持续40天，并用主要的日本雪松花粉过敏原（SBP）使其致敏。在SBP鼻腔激发后5分钟内计数打喷嚏次数。我们分析了SBP特异性免疫球蛋白滴度、肥大细胞蛋白酶1的血清浓度以及用SBP刺激的脾细胞产生的细胞因子。
与施用生理盐水的小鼠相比，施用FPP的小鼠打喷嚏次数显著减少。与生理盐水相比，稀释10倍的FPP也减少了打喷嚏次数，尽管不显著。与生理盐水处理的小鼠相比，摄入FPP的小鼠肥大细胞蛋白酶1的血清水平有被抑制的趋势。各组之间SBP特异性免疫球蛋白滴度和细胞因子产生相当。
我们的结果表明，摄入FPP可减轻JCP症状，而不会改变全身免疫反应。
