NSUN2 缺乏对 HEK293 细胞中 mRNA 5-甲基胞嘧啶修饰和基因表达谱的影响。

Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells.

机构信息

Institute of Epigenetics & Epigenomics & College of Animal Science & Technology, Yangzhou University, 48 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China.

Jiangsu Co-Innovation Center for Prevention & Control of Important Animal Infectious Diseases & Zoonoses, Yangzhou 225009, PR China.

出版信息

Epigenomics. 2019 Feb;11(4):439-453. doi: 10.2217/epi-2018-0169. Epub 2018 Dec 11.

DOI:10.2217/epi-2018-0169

PMID:30526041

Abstract

AIM

To study the biological function of NSUN2 in regulating gene expression and cell proliferation.

MATERIALS & METHODS: The NSUN2 gene was knocked down in HEK293 cells via CRISPR/Cas9 system. mRNA m5C modification and gene expression were assessed using RNA-BisSeq and RNA-Seq.

RESULTS

NSUN2 deficiency could inhibit proliferation and migration of HEK293 cells. A total of 1185 differentially methylated genes and 790 differentially expressed genes were identified. Bioinformatics analysis revealed that the differentially methylated genes were mainly involved in regulating gene expression. Some pathways associated with cell proliferation were significantly enriched by the differentially expressed genes. Additionally, GRB2 and CD44 may be key regulators in NSUN2-mediated cell proliferation.

CONCLUSION

These findings help to elucidate the molecular mechanisms by which NSUN2 affects cell proliferation, migration and other cell phenotypes.

摘要

目的

研究 NSUN2 在调节基因表达和细胞增殖中的生物学功能。

材料与方法

通过 CRISPR/Cas9 系统敲低 HEK293 细胞中的 NSUN2 基因。使用 RNA-BisSeq 和 RNA-Seq 评估 mRNA m5C 修饰和基因表达。

结果

NSUN2 缺陷可抑制 HEK293 细胞的增殖和迁移。共鉴定出 1185 个差异甲基化基因和 790 个差异表达基因。生物信息学分析表明，差异甲基化基因主要参与调节基因表达。一些与细胞增殖相关的通路被差异表达基因显著富集。此外，GRB2 和 CD44 可能是 NSUN2 介导的细胞增殖的关键调节因子。