Garrido-Castañé Ignasi, Romero Anna Ortuño, Espuny Joaquim Castellà, Hentrich Brigitte, Basso Walter
Parasitologia, Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Parasitologia, Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Parasitol Int. 2019 Apr;69:71-74. doi: 10.1016/j.parint.2018.12.001. Epub 2018 Dec 6.
Besnoitia besnoiti is the causative agent of bovine besnoitiosis, a chronic and debilitating disease of cattle that recently re-emerged and seems to be spreading in Europe. A cross-sectional serological study was carried out in different cattle herds in Catalonia, north-eastern Spain, to determine the seroprevalence of B. besnoiti in the region. A total of 791 serum samples (beef cattle n = 338, dairy cattle n = 291; bullfighting cattle n = 162) were tested. Sera were first screened for antibodies against Besnoitia using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) applying a cut-off that was lower than that recommended by the manufacturer in order to reach highest sensitivity. Sera above the chosen cut-off of 15% positivity (PP) were further tested by the Indirect Fluorescent Antibody Test (IFAT) and respectively positive results were confirmed by a B. besnoiti tachyzoite-based immunoblot. A total of 504/791 (63.7%) sera showed ELISA values above the selected cut-off, and 91 of these samples also yielded positive results in IFAT (cut-off titre 1:200). By immunoblot, a positive result was obtained in 93.4% (85 out of the 91) of the IFAT-positive samples. Interestingly, all confirmed Besnoitia-seropositive cases corresponded exclusively to beef cattle from the Pyrenees area, resulting in a prevalence of 25.1% (85/338) at the animal level and of 46% (36/78) at the herd level in this cattle group. No specific antibodies against Besnoitia could be detected in dairy and bullfighting cattle. The obtained results suggested that Besnoitia infections are present in Catalonia, consequently, diagnosis of this parasitic infection should be included in the sanitary control and before trading and movement of animals.
贝氏贝诺孢子虫是牛贝诺孢子虫病的病原体，这是一种影响牛群的慢性消耗性疾病，近期在欧洲再度出现且似有蔓延之势。在西班牙东北部加泰罗尼亚的不同牛群中开展了一项横断面血清学研究，以确定该地区贝氏贝诺孢子虫的血清阳性率。共检测了791份血清样本（肉牛338份、奶牛291份、斗牛162份）。首先使用商用酶联免疫吸附测定法（ELISA）筛查血清中针对贝诺孢子虫的抗体，采用的临界值低于制造商推荐值，以实现最高灵敏度。ELISA检测阳性率（PP）高于选定临界值15%的血清，进一步通过间接荧光抗体试验（IFAT）检测，IFAT检测呈阳性的结果再通过基于贝氏贝诺孢子虫速殖子的免疫印迹法进行确认。791份血清中共有504份（63.7%）ELISA值高于选定临界值，其中91份样本IFAT检测也呈阳性（临界滴度1:200）。通过免疫印迹法，IFAT检测呈阳性的样本中有93.4%（91份中的85份）结果为阳性。有趣的是，所有确诊的贝诺孢子虫血清阳性病例均仅来自比利牛斯地区的肉牛，该牛群中动物个体水平的患病率为25.1%（85/338），牛群水平的患病率为46%（36/78）。在奶牛和斗牛中未检测到针对贝氏贝诺孢子虫的特异性抗体。所得结果表明加泰罗尼亚存在贝氏贝诺孢子虫感染，因此，这种寄生虫感染的诊断应纳入卫生控制以及动物交易和移动之前的检查中。
