Programa de Pós-Graduação em Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Belo Horizonte, MG 31270-010, Brazil.
Programa de Pós-Graduação em Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Belo Horizonte, MG 31270-010, Brazil.
Mol Phylogenet Evol. 2019 Mar;132:105-116. doi: 10.1016/j.ympev.2018.11.022. Epub 2018 Dec 5.
Even though Brazil is the world leader in amphibian diversity, a significant part of its richness remains unknown or hidden under cryptic taxa. Here, we used model-based species delimitation in an integrative taxonomic approach, by gathering molecular and morphometric data to assess cryptic taxa within the monkey frogs Pithecopus rohdei, from the Atlantic Forest, and P. megacephalus, from campos rupestres ecosystem. We sampled one mitochondrial, five nuclear loci, and 18 morphometric variables. Using species-delimitation methods with genetic and morphometric data, we recovered five divergent lineages within P. rohdei and no cryptic lineages were recovered for P. megacephalus. Morphometric data show differentiation only for one of the candidate species revealed by the delimitation approaches, suggesting that individuals from Doce River basin constitute a putative species for formal taxonomic description. The time-calibrated mtDNA tree shows that P. rohdei complex lineages began to diverge in late Miocene. However, dates from the multilocus species tree are more recent, occurring in Pleistocene, and suggesting their persistence in refuges of forest and sky islands within the Atlantic Forest biome. The existence of cryptic taxa within P. rohdei is, therefore, relevant for planning conservation strategies for this species complex in the Atlantic Forest biodiversity hotspot.
尽管巴西是世界上两栖动物多样性的领导者，但它的丰富物种仍有很大一部分未知或隐藏在隐种中。在这里，我们使用基于模型的物种划分方法，结合分子和形态数据，评估来自大西洋森林的猴蛙 Pithecopus rohdei 和来自岩石草原生态系统的 P. megacephalus 中的隐种。我们采集了一个线粒体、五个核基因座和 18 个形态变量的数据。使用遗传和形态数据的物种划分方法，我们在 P. rohdei 中恢复了五个分歧的谱系，而在 P. megacephalus 中没有发现隐种谱系。形态数据仅显示出通过划分方法揭示的候选物种之一的分化，这表明来自多塞河盆地的个体构成了一个有待正式分类描述的假定物种。时间校准的 mtDNA 树表明，P. rohdei 复杂谱系在中新世晚期开始分化。然而，来自多基因座物种树的日期更新近，发生在更新世，表明它们在大西洋森林生物多样性热点地区的森林和天空岛屿避难所中得以持续存在。因此，P. rohdei 中的隐种存在对该物种复杂在大西洋森林的保护策略规划具有重要意义。
