Inoué S
Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
Cell Motil Cytoskeleton. 1988;10(1-2):13-7. doi: 10.1002/cm.970100105.
The progress in video microscopy is reviewed from its early inception, especially with respect to improvements of the microscope image quality. Very recent advances that provide serial optical sections and depth of field as thin as 0.1 micron and that make possible the recording of birefringent images of individual microtubules (25 nm in diameter) directly in live, dividing cells are also documented.
本文回顾了视频显微镜从早期创立以来的发展历程,尤其着重于显微镜图像质量的提升。文中还记录了一些最新进展,这些进展能够提供连续光学切片,景深薄至0.1微米,并且能够直接在活的分裂细胞中记录直径为25纳米的单个微管的双折射图像。