College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China; Sichuan National Inspection and Testing Co., Ltd., Luzhou, Sichuan 646000, PR China.
College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China; Ya'an Polytechnic College, Ya'an, Sichuan 625000, PR China.
Int J Biol Macromol. 2019 Mar 15;125:87-91. doi: 10.1016/j.ijbiomac.2018.12.055. Epub 2018 Dec 5.
In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its K increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application.
本文建立了固定在玻璃纤维膜载体上的未纯化麦麸酯酶的催化性能，观察到的固定化条件为酶 1ml、磷酸盐缓冲液 3ml（pH7.0）、固定化时间 1h、固定化温度 29°C。载体功能化后，研究了固定化酶的一些特性，结果表明固定化酶呈现出比游离酶更好的特性。游离酶和固定化酶的最适 pH 值分别为 8 和 7。游离酶和固定化酶的最适温度分别为 30°C 和 40°C。当酶固定在玻璃纤维膜上时，其 K 值增加了约 7 倍。此外，通过膜固定化，游离麦酯酶的储存和热稳定性得到提高，在储存 12 天后，固定化酶在 4°C 下仍保留约 91.10%的原始活性，表明其在工业应用中具有很大的潜力。
