Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing 211166, China; Jiangsu Center for Safety Evaluation of Drugs, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China.
Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing 211166, China.
J Neurol Sci. 2019 Jan 15;396:247-253. doi: 10.1016/j.jns.2018.11.027. Epub 2018 Nov 22.
Glioblastoma (GBM) is a typical malignant tumor, and there are no effective drugs capable of improving patient survival. Docosahexaenoic acid (DHA), a nutrient essential to animal health and neurodevelopment, exerts an anticancer effect in several types of cancer. However, the function of DHA in GBM is still unclear. Here, we showed that DHA could repress the migration and invasion of GBM U251 cells and promote their apoptosis in a dose- and time-dependent manner, indicating that DHA has an anticancer effect on GBM cells. Whole-transcriptome analysis indicated that DHA treatment mainly regulates the genes associated with receptor binding, oxidoreductase activity, organic acid transmembrane transporter activity, and carboxylic acid transmembrane transporter activity. Long non-coding RNAs (LncRNAs) involved in the regulation network of DHA were also identified, and their targets were assigned to the Gene Ontology (GO) categories. In silico analysis was conducted to predict the pathways related to the differentially expressed genes by DHA treatment. Our findings suggest that DHA acts as an antitumor agent in GBM, which may provide a suitable means of improving the efficacy of GBM treatment in the future.
胶质母细胞瘤（GBM）是一种典型的恶性肿瘤，目前尚无有效药物能够改善患者的生存。二十二碳六烯酸（DHA）是动物健康和神经发育所必需的营养物质，在几种类型的癌症中发挥抗癌作用。然而，DHA 在 GBM 中的作用尚不清楚。在这里，我们表明 DHA 可以以剂量和时间依赖的方式抑制 GBM U251 细胞的迁移和侵袭，并促进其凋亡，这表明 DHA 对 GBM 细胞具有抗癌作用。全转录组分析表明，DHA 处理主要调节与受体结合、氧化还原酶活性、有机酸跨膜转运体活性和羧酸跨膜转运体活性相关的基因。还鉴定了参与 DHA 调节网络的长非编码 RNA（lncRNA），并将其靶标分配到基因本体论（GO）类别中。通过计算机分析预测了 DHA 处理后差异表达基因相关的途径。我们的研究结果表明，DHA 作为 GBM 的抗肿瘤剂，可能为未来提高 GBM 治疗效果提供合适的手段。
