脂质体灵菌红素和编码串联 GCA 核苷酸的质粒通过 ATM/ATR 信号通路减少小胶质细胞和星形胶质细胞中的炎症反应。

Liposomal prodigiosin and plasmid encoding serial GCA nucleotides reduce inflammation in microglial and astrocyte cells by ATM/ATR signaling.

机构信息

Department of Physiology and Pharmacology, Faculty of medicine, Mazandaran University of Medical Science, Sari, Iran.

Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.

出版信息

J Neuroimmunol. 2019 Jan 15;326:75-78. doi: 10.1016/j.jneuroim.2018.11.014. Epub 2018 Dec 1.

DOI:10.1016/j.jneuroim.2018.11.014

PMID:30530109

Abstract

The aim of this study was to use liposomal structure consisting prodigiosin and plasmid encoding serial GCA nucleotides (LP/pSGCAN) to reduce inflammation in microglial cells (MGCs) and astrocyte cells (ACCs) by ATM/ATR signaling. Here, it was shown that LP/pSGCAN decreased cell viability and total RNA level. Importantly, LP/pSGCAN had more effect on ACCs than MGCs (P < 0.05). Moreover, increase of apoptosis was seen with increase of concentration. The expression of IL-1 and IL-6 were decreased and the expression of ATM and ATR were increased in treated MGCs and ACCs, which showed LP/pSGCAN could inhibit inflammation by activation of ATM/ATR pathway.

摘要

本研究旨在利用含有灵菌红素和编码串联 GCA 核苷酸的质粒（LP/pSGCAN）的脂质体结构，通过 ATM/ATR 信号通路减少小胶质细胞（MGCs）和星形胶质细胞（ACCs）中的炎症。结果表明，LP/pSGCAN 降低了细胞活力和总 RNA 水平。重要的是，LP/pSGCAN 对 ACCs 的作用强于 MGCs（P<0.05）。此外，随着浓度的增加，细胞凋亡增加。在处理的 MGCs 和 ACCs 中，IL-1 和 IL-6 的表达减少，ATM 和 ATR 的表达增加，表明 LP/pSGCAN 可以通过激活 ATM/ATR 通路抑制炎症。