Department of Physiology and Pharmacology, Faculty of medicine, Mazandaran University of Medical Science, Sari, Iran.
Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
J Neuroimmunol. 2019 Jan 15;326:75-78. doi: 10.1016/j.jneuroim.2018.11.014. Epub 2018 Dec 1.
The aim of this study was to use liposomal structure consisting prodigiosin and plasmid encoding serial GCA nucleotides (LP/pSGCAN) to reduce inflammation in microglial cells (MGCs) and astrocyte cells (ACCs) by ATM/ATR signaling. Here, it was shown that LP/pSGCAN decreased cell viability and total RNA level. Importantly, LP/pSGCAN had more effect on ACCs than MGCs (P < 0.05). Moreover, increase of apoptosis was seen with increase of concentration. The expression of IL-1 and IL-6 were decreased and the expression of ATM and ATR were increased in treated MGCs and ACCs, which showed LP/pSGCAN could inhibit inflammation by activation of ATM/ATR pathway.
本研究旨在利用含有灵菌红素和编码串联 GCA 核苷酸的质粒（LP/pSGCAN）的脂质体结构，通过 ATM/ATR 信号通路减少小胶质细胞（MGCs）和星形胶质细胞（ACCs）中的炎症。结果表明，LP/pSGCAN 降低了细胞活力和总 RNA 水平。重要的是，LP/pSGCAN 对 ACCs 的作用强于 MGCs（P<0.05）。此外，随着浓度的增加，细胞凋亡增加。在处理的 MGCs 和 ACCs 中，IL-1 和 IL-6 的表达减少，ATM 和 ATR 的表达增加，表明 LP/pSGCAN 可以通过激活 ATM/ATR 通路抑制炎症。
