Department of Parasitology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan, China.
West China School of Public Health, Sichuan University, Chengdu, Sichuan, China.
Antimicrob Agents Chemother. 2019 Feb 26;63(3). doi: 10.1128/AAC.01233-18. Print 2019 Mar.
is one of the most widespread obligatory parasitic protozoa and infects nearly all warm-blooded animals, leading to toxoplasmosis. The therapeutic drugs currently administered, like the combination of pyrimethamine and sulfadiazine, show high rates of toxic side effects, and drug resistance is encountered in some cases. Resveratrol is a natural plant extract with multiple functions, such as antibacterial, anticancer, and antiparasite activities. In this study, we evaluated the inhibitory effects of resveratrol on tachyzoites of the RH strain extracellularly and intracellularly. We demonstrate that resveratrol possesses direct antitoxoplasma activity by reducing the population of extracellularly grown tachyzoites, probably by disturbing the redox homeostasis of the parasites. Moreover, resveratrol was also able to release the burden of cellular stress, promote apoptosis, and maintain the autophagic status of macrophages, which turned out to be regulated by intracellular parasites, thereby functioning indirectly in eliminating In conclusion, resveratrol has both direct and indirect antitoxoplasma effects against RH tachyzoites and may possess the potential to be further evaluated and employed for toxoplasmosis treatment.
弓形虫是最广泛流行的专性寄生原生动物之一，几乎感染所有温血动物，导致弓形体病。目前使用的治疗药物，如乙胺嘧啶和磺胺嘧啶的联合用药，具有很高的毒副作用发生率，并且在某些情况下会出现耐药性。白藜芦醇是一种具有多种功能的天然植物提取物，具有抗菌、抗癌和抗寄生虫活性。在这项研究中，我们评估了白藜芦醇对 RH 株速殖子的体外和体内抑制作用。我们证明白藜芦醇通过干扰寄生虫的氧化还原平衡，直接抑制体外生长的速殖子的数量，从而具有直接抗弓形虫活性。此外，白藜芦醇还能够减轻细胞应激的负担，促进细胞凋亡，并维持巨噬细胞的自噬状态，而这被证明是由细胞内寄生虫调节的，从而间接地在消除 RH 速殖子方面发挥作用。总之，白藜芦醇对 RH 速殖子具有直接和间接的抗弓形虫作用，可能具有进一步评估和用于弓形体病治疗的潜力。
