von Rüden T, Wagner E F
European Molecular Biology Laboratory, Heidelberg, FRG.
EMBO J. 1988 Sep;7(9):2749-56. doi: 10.1002/j.1460-2075.1988.tb03129.x.
The human epidermal growth factor-receptor (EGF-R) was introduced into primary mouse bone marrow cells (BMC), utilizing retrovirus mediated gene transfer. Cultivation of infected BMC in the presence of interleukin-3 (IL-3) led to the outgrowth of IL-3 dependent myeloid cells, which efficiently expressed functional EGF-R, exhibiting its two characteristic affinity states. EGF acts on these cells synergistically with IL-3 in stimulating DNA synthesis and cell proliferation even under IL-3 saturation conditions. However, EGF was not sufficient to replace the requirement for IL-3. In contrast, EGF was able to maintain proliferation of a factor-dependent hemopoietic cell line (FDC-P1) infected with the EGF-R retrovirus in the absence of IL-3, but these cells did not respond to EGF in the presence of IL-3. No influence of EGF on IL-3 induced mast cell differentiation of BMC expressing the EGF-R could be observed by histological criteria. These data show that the expression of EGF-R alone is not sufficient to induce or maintain cell proliferation in IL-3 dependent bone marrow derived cells, although it can do so in established hemopoietic cell lines.
利用逆转录病毒介导的基因转移技术，将人表皮生长因子受体（EGF-R）导入原代小鼠骨髓细胞（BMC）。在白细胞介素-3（IL-3）存在的情况下培养受感染的BMC，导致IL-3依赖性髓系细胞生长，这些细胞高效表达功能性EGF-R，呈现出其两种特征性亲和状态。即使在IL-3饱和条件下，EGF与IL-3协同作用于这些细胞，刺激DNA合成和细胞增殖。然而，EGF不足以替代对IL-3的需求。相反，在没有IL-3的情况下，EGF能够维持感染了EGF-R逆转录病毒的因子依赖性造血细胞系（FDC-P1）的增殖，但这些细胞在有IL-3的情况下对EGF无反应。通过组织学标准未观察到EGF对表达EGF-R的BMC的IL-3诱导的肥大细胞分化有影响。这些数据表明，仅EGF-R的表达不足以在IL-3依赖性骨髓来源细胞中诱导或维持细胞增殖，尽管它可以在已建立的造血细胞系中做到这一点。
