Mottolese M, Venturo I, Donnorso R P, Curcio C G, Rinaldi M, Natali P G
Istituto Nazionale Tumori Regina Elena, Roma, Italy.
Eur J Cancer Clin Oncol. 1988 Aug;24(8):1277-84. doi: 10.1016/0277-5379(88)90215-5.
While conventional cytodiagnosis can, in most instances, recognize cancer cells in metastatic effusions from solid tumors, the cellular type or the organ of origin of the primary neoplasia can rarely be determined only on the basis of their morphology. In the present study we have evaluated whether immunocytochemical techniques can be used to overcome this limitation by employing a panel of monoclonal antibodies (MoAbs) to tumor associated antigens (TAA) which lack detectable reactivity with mesothelial cells. To this end we have analyzed, by indirect immunofluorescence, cytospins of 60 malignant effusions of unknown origin. The results of this study have shown that the definition of the origin of the primary tumor, which was subsequently confirmed histologically and/or clinically, could be reached in 87% of the cases. These findings demonstrate that selected combinations of MoAbs, when used in immunocytochemical tests, can provide a powerful diagnostic tool in defining the site of cryptic primary neoplasias causing metastatic effusions.
虽然传统的细胞诊断在大多数情况下能够识别实体瘤转移积液中的癌细胞，但仅根据其形态很少能确定原发性肿瘤的细胞类型或起源器官。在本研究中，我们评估了免疫细胞化学技术是否可通过使用一组针对肿瘤相关抗原（TAA）的单克隆抗体（MoAb）来克服这一局限性，这些抗原与间皮细胞缺乏可检测到的反应性。为此，我们通过间接免疫荧光分析了60例不明来源恶性积液的细胞涂片。本研究结果表明，在87%的病例中能够确定原发性肿瘤的起源，随后经组织学和/或临床证实。这些发现表明，在免疫细胞化学检测中使用选定的单克隆抗体组合，可为确定导致转移性积液的隐匿性原发性肿瘤的部位提供强大的诊断工具。
