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基于昆虫气味受体的生物传感器的制备与表征数据。

Data on preparation and characterization of an insect odorant receptor based biosensor.

作者信息

Khadka Roshan, Aydemir Nihan, Carraher Colm, Hamiaux Cyril, Colbert Damon, Cheema Jamal, Malmström Jenny, Kralicek Andrew, Travas-Sejdic Jadranka

机构信息

Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand.

MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand.

出版信息

Data Brief. 2018 Nov 7;21:2142-2148. doi: 10.1016/j.dib.2018.11.018. eCollection 2018 Dec.

Abstract

Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled "An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants" [1].

摘要

昆虫气味受体(OrXs)可作为生物传感器中的识别元件,因为它们对挥发性有机化合物表现出高度的敏感性和选择性。在此,我们描述了一种表达和纯化昆虫气味受体并将其重构到人工脂质双层(脂质体)中的方法。这些OrX/脂质体被共价连接到金表面,并使用具有耗散监测功能的石英晶体微天平(QCM-D)进行表征。通过电化学阻抗谱(EIS)和QCM-D研究了固定在金表面的OrX/脂质体与正负气味剂的相互作用。本文所呈现的数据与题为“一种基于超灵敏电化学阻抗的生物传感器,利用昆虫气味受体检测气味剂”的研究论文[1]相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/216f/6265521/a38957f69098/gr1.jpg

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