Guest Paul C
Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Methods Mol Biol. 2019;1916:213-222. doi: 10.1007/978-1-4939-8994-2_20.
This chapter describes the propagation and characterization of transplantable insulinoma cells as model of insulin-producing pancreatic islet cells in the rat. Here, the cells are propagated by transplantation into rats followed by harvesting after growth for approximately 1 month. The cells are then purified by Percoll density gradient centrifugation and characterized by pulse-chase radiolabelling and immunoprecipitation of the insulin-related peptides. The results show that the transplantable insulinoma cells produce insulin in a manner similar to that found in normal pancreatic islets.
本章描述了可移植胰岛素瘤细胞的增殖及特性，该细胞作为大鼠胰岛素分泌性胰岛细胞的模型。在此，通过将细胞移植到大鼠体内，待其生长约1个月后进行收获，以此来增殖细胞。然后，通过Percoll密度梯度离心法对细胞进行纯化，并通过脉冲追踪放射性标记和胰岛素相关肽的免疫沉淀对其进行特性分析。结果表明，可移植胰岛素瘤细胞产生胰岛素的方式与正常胰岛中的情况相似。
