Casaro Mateus, Souza Vanessa R, Oliveira Fernando A, Ferreira Caroline M
Department of Pharmaceutical Sciences, Institute of Environmental, Chemistry and Pharmaceutical Sciences, Universidade Federal de São Paulo, Diadema, SP, Brazil.
Cellular and Molecular Neurobiology Laboratory (LaNeC), Center for Mathematics, Computing and Cognition (CMCC), Federal University of ABC-UFABC, São Bernardo do Campo, SP, Brazil.
Methods Mol Biol. 2019;1916:297-301. doi: 10.1007/978-1-4939-8994-2_28.
Asthma is a worldwide public health issue, affecting the sufferer's quality of life. Many researchers are extensively studying the cellular processes involved in the affected airways. Experimental asthma using animals has been performed for a long time, mainly applying murine models due to well-known advantages. The aim of this study is to present an allergic airway inflammation protocol in mice. Basically, the allergic airway inflammation is induced by intraperitoneal sensitization and intratracheal challenge with ovalbumin (OVA). The model provided here mimics acute asthma characteristics including excessive mucus production, airway hyperresponsiveness, and eosinophilic airway inflammation.
哮喘是一个全球性的公共卫生问题，影响着患者的生活质量。许多研究人员正在广泛研究受影响气道中涉及的细胞过程。使用动物进行实验性哮喘研究已经开展了很长时间，主要由于众所周知的优势而应用小鼠模型。本研究的目的是介绍一种小鼠过敏性气道炎症方案。基本上，过敏性气道炎症是通过腹腔致敏和用卵清蛋白（OVA）进行气管内激发来诱导的。这里提供的模型模拟了急性哮喘的特征，包括过多的黏液产生、气道高反应性和嗜酸性粒细胞性气道炎症。
