Carpio Marcos A, Katz Samuel G
Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
CIQUIBIC-Department of Biological Chemistry, National University of Cordoba, Cordoba, Argentina.
Methods Mol Biol. 2019;1877:173-183. doi: 10.1007/978-1-4939-8861-7_12.
BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.
BCL-2家族成员在细胞凋亡中除了直接调控线粒体外膜通透性（MOMP）外，还有其他作用。其中一个重要功能是从内质网（ER）释放钙，这对细胞凋亡过程起着关键作用。在此，我们描述了一种测量内质网、线粒体和细胞质中钙水平的方法，并特别考虑了BCL-2家族生物学特性。
