Defo Deeh Patrick Brice, Watcho Pierre, Wankeu-Nya Modeste, Ngadjui Esther, Usman Umar Zayyanu
Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon.
Department of Biophysics, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
Andrologia. 2019 Apr;51(3):e13216. doi: 10.1111/and.13216. Epub 2018 Dec 10.
This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca ] ), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL-97 (R2C) tumour Leydig cells. The cells were divided into nine groups and treated as follows: (a)-Control, (b)-Capsazepine (CPZ, 0.1 mM, a TRPV1 channel blocker), (c)-Capsaicin (CAP, 0.01 mM, a TRPV1 channel activator), (d)-GT (500 μg/ml), (e)-GT+CPZ, (f)-GT+CAP, (g)-Se (200 nM), (h)-Se+CPZ and (i)-Se+CAP. After treatments, cell viability, [Ca ] , apoptosis, caspase 3/9, reactive oxygen species (ROS) and mitochondrial membrane depolarisation (MMD) were evaluated. The [Ca ] , apoptosis, caspase 3/9, MMD and ROS levels were significantly (p < 0.001) increased in CAP group, but lowered in CPZ group. Interestingly, these parameters were significantly (p < 0.001) improved by GT and Se, compared to the CAP group. Moreover, the co-administration of GT+CAP or Se+CAP inhibited the cytotoxicity of CAP. Thus, the modulatory properties of GT and Se on Ca influx, apoptosis and oxidative stress require the integrity of TRPV1 channel in CCL-97 Leydig cells. These results suggest that GT and Se might be used in the management of cytotoxicity in the testes, involving TRPV1 channel activity.
本研究通过瞬时受体电位香草酸受体1（TRPV1）通道活性，评估了特斯曼古夷苏木（GT）甲醇提取物和硒（Se）对CCL - 97（R2C）肿瘤莱迪希细胞的细胞活力、细胞内钙浓度（[Ca]）、细胞凋亡和氧化应激的影响。细胞被分为九组，并进行如下处理：（a）-对照组，（b）-辣椒平（CPZ，0.1 mM，一种TRPV1通道阻滞剂），（c）-辣椒素（CAP，0.01 mM, 一种TRPV1通道激活剂），（d）-GT（500 μg/ml），（e）-GT + CPZ，（f）-GT + CAP，（g）-Se（200 nM），（h）-Se + CPZ和（i）-Se + CAP。处理后，评估细胞活力、[Ca]、细胞凋亡、半胱天冬酶3/9、活性氧（ROS）和线粒体膜去极化（MMD）。CAP组中[Ca]、细胞凋亡、半胱天冬酶3/9、MMD和ROS水平显著（p < 0.001）升高，而CPZ组中则降低。有趣的是，与CAP组相比，GT和Se使这些参数显著（p < 0.001）改善。此外，GT + CAP或Se + CAP联合给药可抑制CAP的细胞毒性。因此，GT和Se对钙内流、细胞凋亡和氧化应激的调节特性需要CCL - 九七莱迪希细胞中TRPV1通道的完整性。这些结果表明，GT和Se可能用于涉及TRPV1通道活性的睾丸细胞毒性管理。
