Carlisle K S, Reagan C R, Hersey S J
Gastroenterology. 1978 Mar;74(3):504-10.
The involvement of de novo protein synthesis in acid secretion by the in vitro bullfrog gastric mucosa was examined using the inhibitors cycloheximide and puromycin. Both inhibitors reduced [3H]leucine incorporation by more than 90%. The inhibition of protein synthesis had no influence on spontaneous acid secretion nor did it effect secretory stimulation by histamine, carbachol, pentagastrin, or theophylline. Oxygen consumption by nonstimulated and theophyllinestimulated tissues was shown to be independent of protein synthesis. The dramatic morphological changes observed in oxyntic cells during a transition from rest to active secretion persisted in cycloheximide-and puromycin-treated tissues. Stereological analysis of the apical membrane surface area density failed to demonstrate any quantitative influence of protein synthesis inhibition. These studies quantitatively confirm that protein synthesis is not required for the biochemical and morphological events involved in acid secretion by the in vitro frog gastric mucosa.
使用抑制剂环己酰亚胺和嘌呤霉素检测了体外牛蛙胃黏膜中从头合成蛋白质在胃酸分泌中的作用。两种抑制剂均使[3H]亮氨酸掺入减少了90%以上。蛋白质合成的抑制对自发胃酸分泌没有影响，对组胺、卡巴胆碱、五肽胃泌素或茶碱引起的分泌刺激也没有影响。未受刺激和茶碱刺激的组织的耗氧量显示与蛋白质合成无关。在从静止分泌转变为活跃分泌的过程中，在壁细胞中观察到的显著形态变化在经环己酰亚胺和嘌呤霉素处理的组织中持续存在。对顶端膜表面积密度的体视学分析未能证明蛋白质合成抑制有任何定量影响。这些研究定量证实，体外青蛙胃黏膜胃酸分泌所涉及的生化和形态学事件不需要蛋白质合成。
