衰老影响循环中miR-33a和miR-33b水平与胰岛素抵抗及肥胖的关系。

Ageing influences the relationship of circulating miR-33a and miR- 33b levels with insulin resistance and adiposity.

作者信息

Corona-Meraz Fernanda-Isadora, Vázquez-Del Mercado Mónica, Ortega Francisco José, Ruiz-Quezada Sandra-Luz, Guzmán-Ornelas Milton-Omar, Navarro-Hernández Rosa-Elena

机构信息

1 Instituto de Investigación en Reumatología y del Sistema Musculo Esquelético, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México.

2 UDG-CA-701, Grupo de Investigación Inmunometabolismo en Enfermedades Emergentes, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México.

出版信息

Diab Vasc Dis Res. 2019 May;16(3):244-253. doi: 10.1177/1479164118816659. Epub 2018 Dec 11.

DOI:10.1177/1479164118816659

PMID:30537863

Abstract

OBJECTIVE

The identification of circulating microRNAs related to abnormal metabolic function may be useful in the context of ageing, adiposity and insulin resistance. The miR-33 a/b has been shown to control the expression of genes involved in fatty acid biosynthesis, impaired metabolism and insulin resistance. In this study, we aimed to identify differences in circulating miR-33 a/b levels according to age-related metabolic impairment and increased adiposity.

METHODS

This study included 80 individuals (30.2% with obesity, 70% females) classified according to insulin resistance (Stern's criteria) and age [young (20-39 years) and senior (40-59 years)]. Body fat was evaluated using bioelectrical impedance, biochemical markers by colorimetric, enzymatic and immuno-turbidimetry methods. TaqMan measures of circulating miR-33 a and miR-33 b with quantitative reverse transcription polymerase chain reaction in serum were assessed in association with clinical outputs.

RESULTS

Circulating miR-33 a and miR-33 b levels showed significant association with fatness, the lipid profile and biomarkers of impaired glucose metabolism. Both miR-33 a and miR-33 b were associated with visceral adiposity index in non-insulin resistance and insulin resistance individuals. More important, for miR-33 a circulating levels in senior group, receiver operating characteristic curve analyses showed area under the curve 0.804 ( p = 0.010; 95% confidence interval = 0.655-0.952).

CONCLUSION

Ageing influenced the relationship of circulating miR-33 a and miR-33 b with insulin resistance and increased adiposity.

摘要

目的

鉴定与异常代谢功能相关的循环微小RNA可能有助于了解衰老、肥胖和胰岛素抵抗。已证明miR-33 a/b可控制参与脂肪酸生物合成、代谢受损和胰岛素抵抗的基因表达。在本研究中，我们旨在根据与年龄相关的代谢损伤和肥胖增加来确定循环miR-33 a/b水平的差异。

方法

本研究纳入了80名个体（30.2%患有肥胖症，70%为女性），根据胰岛素抵抗（斯特恩标准）和年龄[年轻（20 - 39岁）和年长（40 - 59岁）]进行分类。使用生物电阻抗评估体脂，通过比色法、酶法和免疫比浊法检测生化指标。采用TaqMan法通过定量逆转录聚合酶链反应测定血清中循环miR-33 a和miR-33 b，并与临床指标相关联进行评估。

结果

循环miR-33 a和miR-33 b水平与肥胖、血脂谱以及葡萄糖代谢受损的生物标志物显著相关。在非胰岛素抵抗和胰岛素抵抗个体中，miR-33 a和miR-33 b均与内脏脂肪指数相关。更重要的是，对于老年组中miR-33 a的循环水平，受试者工作特征曲线分析显示曲线下面积为0.804（p = 0.010；95%置信区间 = 0.655 - 0.952）。