印度泰米尔纳德邦绵羊和山羊物种的形态学与分子鉴定
Morphological and molecular identification of species in sheep and goat at Tamil Nadu, India.
作者信息
Basnett Kanta, Nagarajan K, Soundararajan C, Vairamuthu S, Rao Ganne Venkata Sudhakar
机构信息
1Department of Veterinary Pathology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600007 India.
2Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600007 India.
出版信息
J Parasit Dis. 2018 Dec;42(4):604-607. doi: 10.1007/s12639-018-1042-7. Epub 2018 Oct 13.
A total of 281 fresh fecal samples (65 sheeps and 216 goats) were examined for the presence of species in Tamil Nadu from October 2017 to April 2018. All the faecal samples were examined by direct smear method, saturated sucrose floatation technique and modified Ziehl-Neelsen staining method and it was confirmed-at genus level with PCR technique. Overall prevalence of infection in-small ruminant was 2.14%. The prevalence of species in sheep and goat was 3.08 and 1.85% respectively. oocyst was observed mostly during winter season (Dec-Feb) with 7.14% followed by summer (March and April) and Northeast mansoon (October and November) with 1.67 and 1.66% respectively. The oocyst detected from faecal sample through conventional faecal examination was confirmed by PCR.
2017年10月至2018年4月期间，在泰米尔纳德邦共检测了281份新鲜粪便样本（65份绵羊样本和216份山羊样本），以检测其中的[具体物种]。所有粪便样本均采用直接涂片法、饱和蔗糖漂浮技术和改良齐尔-尼尔森染色法进行检测，并通过聚合酶链反应（PCR）技术在属水平上进行确认。小型反刍动物中[感染物种]的总体患病率为2.14%。绵羊和山羊中[该物种]的患病率分别为3.08%和1.85%。卵囊大多在冬季（12月至2月）被观察到，患病率为7.14%，其次是夏季（3月和4月）和东北季风季节（10月和11月），患病率分别为1.67%和1.66%。通过常规粪便检查从粪便样本中检测到的卵囊通过PCR得到了确认。
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