三磷酸腺苷诱导分离的基体光散射变化。
Adenosine triphosphate induced change in light scattering of isolated basal bodies.
作者信息
Anderson R G, Floyd A K
出版信息
Cell Biol Int Rep. 1978 Sep;2(5):487-94. doi: 10.1016/0309-1651(78)90101-7.
Suspensions of isolated basal bodies undergo a characteristic decrease in turbidity following their exposure to 10(-3) M ATP. Typically, turbidity changes range from 8% to 20%, depending on the preparation, with an average change of 12%. Nucleotides other than ATP did not cause a turbidity decrease. The reaction has a pH optimum of pH 8.5 and is inhibited by concentrations of divalent cations greater than 2 X 10(-3) M. These results indicate that ATP induces a conformational change in the basal body that may be related to its activity in the cell.
分离出的基体悬浮液在暴露于10⁻³ M ATP后，浊度会出现典型的下降。根据制备情况，浊度变化范围为8%至20%，平均变化为12%。除ATP外的其他核苷酸不会导致浊度下降。该反应的最适pH为8.5，并且当二价阳离子浓度大于2×10⁻³ M时会受到抑制。这些结果表明，ATP会诱导基体发生构象变化，这可能与其在细胞中的活性有关。
Zotero 插件