Dou Tong-Yi, Chen Jing, Hao Yi-Fu, Qi Xiaohui
School of Life Science and Medicine, Dalian University of Technology, Dagong Road No. 2, LiaoDongWan New District, Panjin, 124221, China.
College of Life Science, Dalian Minzu University, Dalian, 116600, China.
Arch Microbiol. 2019 Mar;201(2):163-170. doi: 10.1007/s00203-018-1606-z. Epub 2018 Dec 11.
The hemicellulolytic enzyme system produced by Cellulosimicrobium cellulans strain F16 was resolved by ultracentrifugation and size exclusion chromatography. The particle size and molecular weight were determined by both dynamic light scattering and negative stain electron microscopy. The results showed that xylanosomes produced by strain F16 were found to have an apparent sedimentation coefficient of 28 S, were diverse in size (18-70 nm), molecular weight (11-78 MDa) and morphology, but resembled in subunit composition (SDS-PAGE and proteomic results). It is proposed that particles of 22 nm may be the basic unit, while 43 nm and 60 nm particles observed may be dimer and trimer of the basic unit, or xylanosomes with smaller size might be degradation products of larger size xylanosomes. Moreover, such xylanosomes are also found to have strong binding affinity toward water-insoluble substrates such as Avicel, birchwood xylan, and corn cob.
纤维素微杆菌F16菌株产生的半纤维素酶系统通过超速离心和尺寸排阻色谱法进行分离。通过动态光散射和负染电子显微镜测定颗粒大小和分子量。结果表明，F16菌株产生的木聚糖体的表观沉降系数为28 S，大小（18 - 70 nm）、分子量（11 - 78 MDa）和形态各异，但亚基组成相似（SDS - PAGE和蛋白质组学结果）。有人提出，22 nm的颗粒可能是基本单元，而观察到的43 nm和60 nm颗粒可能是基本单元的二聚体和三聚体，或者较小尺寸的木聚糖体可能是较大尺寸木聚糖体的降解产物。此外，还发现这种木聚糖体对水不溶性底物如微晶纤维素、桦木木聚糖和玉米芯具有很强的结合亲和力。
