Ghosh Joydeep, Mohamad Safa F, Srour Edward F
Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
Methods Mol Biol. 2019;2002:181-193. doi: 10.1007/7651_2018_196.
Hematopoietic stem cells (HSCs) are regulated by multiple components of the hematopoietic niche, including bone marrow-derived macrophages and osteomacs. However, both macrophages and osteomacs are phenotypically similar. Thus, specific phenotypic markers are required to differentially identify the effects of osteomacs and bone marrow macrophages on different physiological processes, including hematopoiesis and bone remodeling. Here, we describe a protocol for isolation of murine bone marrow-derived macrophages and osteomacs from neonatal and adult mice and subsequent identification by multi-parametric flow cytometry using an 8-color antibody panel.
造血干细胞（HSCs）受造血微环境的多种成分调控，包括骨髓来源的巨噬细胞和骨巨噬细胞。然而，巨噬细胞和骨巨噬细胞在表型上相似。因此，需要特定的表型标记物来区分骨巨噬细胞和骨髓巨噬细胞对不同生理过程（包括造血和骨重塑）的影响。在这里，我们描述了一种从新生小鼠和成年小鼠中分离骨髓来源的巨噬细胞和骨巨噬细胞，随后使用8色抗体组合通过多参数流式细胞术进行鉴定的方案。
