Ch'ng Angela Chiew Wen, Ahmad Azimah, Konthur Zoltán, Lim Theam Soon
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Methods Mol Biol. 2019;1904:377-400. doi: 10.1007/978-1-4939-8958-4_18.
Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate. The advantage of using a magnetic particle processor system is the ability to carry out phage display panning against multiple target antigens simultaneously in parallel. The system carries out the panning procedure using magnetic nanoparticles in microtiter plates. The entire incubation, wash, and elution process is then automated in this setup. The system also allows customization for the introduction of different panning stringencies. The nature of the biopanning process coupled with the limitation of the system means that minimal human intervention is required for the infection and phage packaging stage. However, the process still allows for rapid and reproducible antibody generation to be carried out.
淘选是从噬菌体抗体库中选择抗体的常用方法。为了实现类似目的，人们开发了几种方法，即链霉亲和素质谱免疫分析（MSIA™）一次性自动化研究提示、磁珠、聚苯乙烯免疫管和微量滴定板。使用磁性粒子处理器系统的优点是能够同时对多种靶抗原进行平行噬菌体展示淘选。该系统在微量滴定板中使用磁性纳米颗粒进行淘选程序。然后在该设置中自动完成整个孵育、洗涤和洗脱过程。该系统还允许定制不同的淘选严格度。生物淘选过程的性质以及系统的局限性意味着在感染和噬菌体包装阶段所需的人工干预最少。然而，该过程仍然能够快速且可重复地生成抗体。
