通过基于细胞的免疫荧光细胞化学法检测cN1A自身抗体。

Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry.

作者信息

Yamashita Satoshi, Tawara Nozomu

机构信息

Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.

出版信息

Methods Mol Biol. 2019;1901:89-94. doi: 10.1007/978-1-4939-8949-2_7.

DOI:10.1007/978-1-4939-8949-2_7

PMID:30539570

Abstract

Sporadic inclusion body myositis (sIBM) is a chronic and progressive inflammatory myopathy that is the commonest among population over 50s. Recently, autoantibodies against cytosolic 5'-nucleotidase 1A (cN1A) have been identified in plasma and serum samples from patients with sIBM. So far, various methods have been established to detect the anti-cN1A autoantibodies, which showed a clinical utility of detection of the autoantibodies in the diagnosis of sIBM. Here we describe a novel cell-based assay for detection of the autoantibodies, which seems equivalent in sensitivity and better in specificity for the diagnosis of sIBM, compared with the previous methods.

摘要

散发性包涵体肌炎（sIBM）是一种慢性进行性炎性肌病，在50岁以上人群中最为常见。最近，在sIBM患者的血浆和血清样本中发现了针对胞质5'-核苷酸酶1A（cN1A）的自身抗体。到目前为止，已经建立了多种方法来检测抗cN1A自身抗体，这些方法显示出在sIBM诊断中检测自身抗体的临床实用性。在此，我们描述了一种用于检测自身抗体的新型细胞检测方法，与先前的方法相比，该方法在诊断sIBM时似乎具有相同的敏感性和更好的特异性。