Ibitoye O B, Ajiboye T O
Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Medical Biochemistry, College of Health Sciences, Nile University of Nigeria, Nigeria.
Microb Pathog. 2019 Feb;127:239-245. doi: 10.1016/j.micpath.2018.12.012. Epub 2018 Dec 9.
(+)-Catechin is a versatile compound with its pharmacophore (catechol and resorcinol) responsible for prooxidant and antibacterial activities. In this study, we present that demonstrating the synergistic interaction between (+)-catechin and quinolone-based antibiotics, ciprofloxacin and gemifloxacin is related to reactive oxygen species generation. The minimum inhibitory concentration of (+)-catechin against Acinetobacter baumannii AB5075 were considerably lowered for ΔsodB and ΔkatG mutants. Checkerboard assay shows synergistic interactions between (+)-catechin and quinolones. (+)-Catechin amplified quinolones-induced redox imbalance by increasing superoxide ion generation, NAD+/NADH ratio and ADP/ATP ratio. Conversely, the level of reduced glutathione was significantly lowered. We conclude that (+)-catechin potentiates quinolone-based antibiotics-induced oxidative stress in A. baumannii by increasing ROS generation, energy metabolism and electron transport chain activity with a concomitant decrease in glutathione.
（+）-儿茶素是一种多功能化合物，其药效基团（儿茶酚和间苯二酚）具有促氧化和抗菌活性。在本研究中，我们发现（+）-儿茶素与喹诺酮类抗生素环丙沙星和吉米沙星之间的协同相互作用与活性氧的产生有关。对于ΔsodB和ΔkatG突变体，（+）-儿茶素对鲍曼不动杆菌AB5075的最低抑菌浓度显著降低。棋盘法显示（+）-儿茶素与喹诺酮类药物之间存在协同相互作用。（+）-儿茶素通过增加超氧阴离子的产生、NAD+/NADH比值和ADP/ATP比值，放大了喹诺酮类药物诱导的氧化还原失衡。相反，还原型谷胱甘肽水平显著降低。我们得出结论，（+）-儿茶素通过增加活性氧的产生、能量代谢和电子传递链活性，并伴随谷胱甘肽的减少，增强了喹诺酮类抗生素对鲍曼不动杆菌诱导的氧化应激。
