从妊娠中期人胎盘中分离绒毛细胞滋养层。
Isolation of villous cytotrophoblasts from second trimester human placentas.
机构信息
Robinson Research Institute, University of Adelaide, Adelaide, SA, 5005, Australia; Adelaide Medical School, University of Adelaide, Adelaide, SA, 5000, Australia.
Robinson Research Institute, University of Adelaide, Adelaide, SA, 5005, Australia; Adelaide Medical School, University of Adelaide, Adelaide, SA, 5000, Australia.
出版信息
Placenta. 2018 Dec 15;74:55-58. doi: 10.1016/j.placenta.2018.11.007. Epub 2018 Nov 22.
Human placental functional studies are often performed using immortalised trophoblast cells and cell lines established from human choriocarcinomas, which, while practical, may not truly reflect trophoblast function in vivo. Primary trophoblast cultures derived from human placentas following pregnancy termination or delivery are more clinically relevant, but trophoblast isolation protocols are only available for 1st trimester and term placental tissues. Here we report a method for isolation and purification of primary villous cytotrophoblasts from 2nd trimester human placentas, that yields >99% trophoblast purity as shown by immunofluorescence.
人类胎盘功能研究通常使用永生化滋养层细胞和源自人绒毛膜癌的细胞系进行，虽然实用，但可能无法真实反映体内滋养层功能。从终止妊娠或分娩后的人胎盘获得的原代滋养层培养物更具有临床相关性，但滋养层分离方案仅适用于 1 期和足月胎盘组织。在这里，我们报告了一种从 2 期人胎盘分离和纯化原代绒毛滋养细胞的方法，如免疫荧光所示，该方法可获得 >99%的滋养层纯度。
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