Jeschke Udo, Richter D U, Hammer A, Briese V, Friese K, Karsten U
Department of Obstetrics and Gynaecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany,
Histochem Cell Biol. 2002 Mar;117(3):219-26. doi: 10.1007/s00418-002-0383-5. Epub 2002 Feb 9.
The Thomsen-Friedenreich (TF) antigen (or, more precisely, epitope Galbeta1-3GalNAcalpha-O-) has been known for a long time as a carcinoma-associated antigen. In normal tissues the occurrence of TF antigen is restricted to a few immunologically privileged areas. Here we report on the identification of the TF epitope and its putative carrier protein mucin 1 (MUC1) in human placental tissue, on isolated trophoblast cells in vitro and on trophoblast tumour cell lines BeWo and Jeg3. Cryosections of placental and decidual tissues of the first, second and third trimester were double stained with monoclonal antibodies directed against the TF epitope (IgM) and against MUC1 (IgG). In the first trimester of pregnancy we found strong expression of TF antigen and MUC1 at the apical side of the syncytiotrophoblast directed towards the maternal blood. This expression was consistent in the second trimester of pregnancy, and to a lesser degree in the third trimester. In addition, we found positive staining for TF antigen and MUC1 on extravillous trophoblast cells in the decidua during the first and second trimester of pregnancy. Trophoblast tumour cells of the cell line BeWo, which form a syncytium in vitro, were also positive for TF antigen and MUC1, whereas Jeg3 cells, which are unable to form a syncytium, expressed only MUC1. Freshly isolated trophoblast cells from first trimester placentas showed strong staining for MUC1; however, only a few of these cells (less than 1%) were positive for TF antigen, and might consist of digested fragments of the syncytium. In summary, TF antigen and MUC1 are expressed by the syncytiotrophoblast at the feto-maternal interface and by extravillous trophoblast cells invading the decidua, whereas villous cytotrophoblast cells in situ as well as freshly isolated trophoblast cells from first trimester placentas only express MUC1 but not TF antigen.
汤姆森 - 弗里德赖希（TF）抗原（或者更准确地说，表位Galβ1-3GalNAcα - O - ）长期以来一直被认为是一种癌相关抗原。在正常组织中，TF抗原的出现仅限于少数免疫特权区域。在此，我们报告了在人胎盘组织、体外分离的滋养层细胞以及滋养层肿瘤细胞系BeWo和Jeg3中TF表位及其假定的载体蛋白粘蛋白1（MUC1）的鉴定。用针对TF表位（IgM）和针对MUC1（IgG）的单克隆抗体对妊娠第一、第二和第三个月的胎盘和蜕膜组织冰冻切片进行双重染色。在妊娠第一个月，我们发现合体滋养层细胞朝向母体血液的顶端侧有强烈的TF抗原和MUC1表达。这种表达在妊娠第二个月是一致的，在妊娠第三个月程度较轻。此外，我们发现妊娠第一和第二个月蜕膜中绒毛外滋养层细胞上TF抗原和MUC1呈阳性染色。在体外形成合体的细胞系BeWo的滋养层肿瘤细胞也对TF抗原和MUC1呈阳性，而不能形成合体的Jeg3细胞仅表达MUC1。从妊娠第一个月胎盘新鲜分离的滋养层细胞对MUC1有强烈染色；然而，这些细胞中只有少数（不到1%）对TF抗原呈阳性，可能由合体的消化片段组成。总之，TF抗原和MUC1由母胎界面的合体滋养层细胞以及侵入蜕膜的绒毛外滋养层细胞表达，而原位绒毛细胞滋养层细胞以及从妊娠第一个月胎盘新鲜分离的滋养层细胞仅表达MUC1而不表达TF抗原。
