The marine Gram-negative bacterium Novosphingobium sp. PP1Y as a potential source of novel metabolites with antioxidant activity.
作者信息
Petruk Ganna, Roxo Mariana, De Lise Federica, Mensitieri Francesca, Notomista Eugenio, Wink Michael, Izzo Viviana, Monti Daria Maria
机构信息
Department of Chemical Sciences, University Federico II of Naples, Via Cinthia 26, 80127, Naples, Italy.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120, Heidelberg, Germany.
出版信息
Biotechnol Lett. 2019 Feb;41(2):273-281. doi: 10.1007/s10529-018-02636-4. Epub 2018 Dec 12.
OBJECTIVE
The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated.
RESULTS
The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation. In the case of the cells, pre-treatment with methanolic extract was also able to maintain unaltered intracellular GSH levels and phosphorylation levels of mitogen-activated protein kinases p38. Instead, in the case of the worms, the extract was able to modulate the expression levels of stress response genes, by activating the transcription factor skn-1.
CONCLUSIONS
From a biotechnological and economical point of view, antioxidants from microorganisms are convenient as they provide a valid alternative to chemical synthesis and respond to the ever-growing market demand for natural antioxidants.
Zotero 插件