Tanık Abdulsamet, Güler Doğru Arzum, Akpolat Veysi, Acun Kaya Filiz, Sarıbaş Ebru, Gül Mehmet, İrtegün Kandemir Sevgi, Deveci Engin
Department of Periodontology, Faculty of Dentistry, Adıyaman University, Adıyaman, Turkey
Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakır, Turkey
Turk J Med Sci. 2018 Dec 12;48(6):1302-1314. doi: 10.3906/sag-1803-201.
BACKGROUND/AIM: In this experimental study, we aimed to evaluate the late period effects of the combination of Ankaferd Blood Stopper (ABS), which has bone wound healing effects, and β-tricalcium phosphate (TCP) on the regeneration of bone tissue through histopathological, immunohistochemical, and radiological (dual energy X-ray absorptiometry - DEXA) methods in nondiabetic rats.
Sixty-four Wistar albino male rats were used. In the calvaria of the rats, a bone defect 7.0 mm in diameter was created. These rats were divided into 4 different groups. Group 1 was the control group without any treatment, a 0.125 mL Β-TCP graft was applied to Group 2, a 0.125 mL ABS was applied to Group 3, and a 0.125 mL (β-TCP + ABS) mixture was applied to group 4. Half of the rats were sacrificed on day 28 and the other half on day 56. Histopathological, immunohistochemical, and DEXA analyses of the specimens were performed after the experiment.
As a result of the histopathological analysis, osteoblastic activity and new bone formation were found to be significantly higher in Group 2, Group 3, and Group 4 than the control group on day 28 (P < 0.05). However, inflammatory cell infiltration and vascular dilatation and hemorrhage decreased significantly compared to the control group (P < 0.05). The histopathological analysis in rats on day 56 showed that osteoblastic activity in Group 2 and Group 4 was significantly higher than in the control group, but there was a statistically significant decrease in inflammatory cell infiltration and vascular dilatation and hemorrhage compared to the control group (P < 0.05). New bone formation in Group 2, Group 3, and Group 4 was significantly higher than in the control group. Western blotting findings revealed that the osteonectin and osteopontin expression on day 28 was increased significantly in Group 2 and Group 4. DEXA analyses revealed that BMC values in Group 2 and Group 4 on day 28 were significantly higher than in the control group (P < 0.05). There was no significant difference in bone mineral density values on the 28th and 56th days (P > 0.05).
The use of both β-TCP + ABS and only ABS had positive effects on wound healing and bone formation in nondiabetic rats.
背景/目的：在本实验研究中，我们旨在通过组织病理学、免疫组织化学和放射学（双能X线吸收法 - DEXA）方法，评估具有骨创伤愈合作用的安卡福得止血剂（ABS）与β-磷酸三钙（TCP）联合使用对非糖尿病大鼠骨组织再生的后期影响。
使用64只Wistar白化雄性大鼠。在大鼠颅骨上制造一个直径7.0毫米的骨缺损。这些大鼠被分为4个不同的组。第1组为未进行任何处理的对照组，第2组应用0.125 mL的β-TCP移植物，第3组应用0.125 mL的ABS，第4组应用0.125 mL的（β-TCP + ABS）混合物。一半的大鼠在第28天处死，另一半在第56天处死。实验结束后对标本进行组织病理学、免疫组织化学和DEXA分析。
组织病理学分析结果显示，在第28天，第2组、第3组和第4组的成骨细胞活性和新骨形成明显高于对照组（P < 0.05）。然而，与对照组相比，炎症细胞浸润、血管扩张和出血明显减少（P < 0.05）。第56天大鼠组织病理学分析表明，第2组和第4组成骨细胞活性明显高于对照组，但与对照组相比，炎症细胞浸润、血管扩张和出血在统计学上有显著减少（P < 0.05）。第
