Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, Kunming, Yunnan, China.
First Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
PLoS One. 2018 Dec 13;13(12):e0209014. doi: 10.1371/journal.pone.0209014. eCollection 2018.
Three arsenic species in urine are measured using an atomic absorption spectrophotometer. RT-PCR is performed to detect the expression levels of AS3MT, 3 miRNAs, and 17 relative mRNAs in 43 workers producing arsenic trioxide, 36 workers who stopped exposure to arsenic for 85 days, and 24 individuals as the control group. The concentrations of urinary arsenic are very high in workers. A negative correlation between AS3MT and MiR-548c-3p is found. There exist significant changes for most selected miRNAs and mRNAs in workers. There are no significant differences between workers who stopped exposure to arsenic and the control group for most miRNAs and mRNAs, but the MiR-548c-3p levels show significant changes. Similar positive correlations between the expression of AS3MT and all selected mRNAs are found. Negative correlations between the expression of MiR-548c-3p and many relative mRNAs are found as well. AS3MT and MiR-548c-3p may regulate arsenic methylation jointly, which when involved in a group of relative mRNAs may play roles in arsenic metabolism and epigenetic changes caused by this metabolism.
采用原子吸收分光光度计测定尿中三种砷形态。采用 RT-PCR 检测 43 名生产三氧化二砷工人、36 名停止砷暴露 85 天工人和 24 名对照组个体中 AS3MT、3 种 miRNA 和 17 种相对 mRNA 的表达水平。工人尿砷浓度非常高。发现 AS3MT 与 MiR-548c-3p 呈负相关。大多数选定的 miRNA 和 mRNA 在工人中存在显著变化。大多数 miRNA 和 mRNA 在停止砷暴露的工人和对照组之间没有显著差异，但 MiR-548c-3p 水平显示出显著变化。AS3MT 的表达与所有选定的 mRNA 之间存在相似的正相关。MiR-548c-3p 的表达与许多相对 mRNA 之间存在负相关。AS3MT 和 MiR-548c-3p 可能共同调节砷甲基化，当涉及一组相对 mRNA 时，它们可能在砷代谢和这种代谢引起的表观遗传变化中发挥作用。
