细胞外质粒 DNA 增强药物电转移。

Enhancement of drug electrotransfer by extracellular plasmid DNA.

机构信息

Biophysical Research Group, Vytautas Magnus University, Kaunas, Lithuania.

出版信息

Arch Biochem Biophys. 2019 May 15;666:156-160. doi: 10.1016/j.abb.2018.12.010. Epub 2018 Dec 10.

DOI:10.1016/j.abb.2018.12.010

Abstract

Electroporation is a widely established method for molecular delivery across electric field perturbed plasma membrane. It can be used as a non-viral DNA transfection method, or as a way to achieve small molecule delivery to or extraction from cells. We examined the possibility of combining the DNA delivery to the cells with small molecule transport across electroporated plasma membrane. The results show that the presence of DNA in electroporation medium increases the extraction of fluorescent dye calcein from calcein-AM loaded cells as well as the delivery of small-molecule drug bleomycin to the cells. We propose that these results may have implications in enhanced drug delivery using electroporation both in vivo and in clinics.

摘要

电穿孔是一种广泛应用于通过电场干扰的质膜进行分子传递的方法。它可用作非病毒 DNA 转染方法，或用于实现小分子向细胞内或从细胞内的输送。我们研究了将 DNA 递送到细胞与小分子通过电穿孔质膜运输相结合的可能性。结果表明，电穿孔介质中 DNA 的存在会增加从 calcein-AM 负载细胞中提取荧光染料 calcein 的量，以及将小分子药物博来霉素递送到细胞内的量。我们提出，这些结果可能对使用电穿孔在体内和临床中增强药物输送具有重要意义。