Search of Allosteric Inhibitors and Associated Proteins of an AKT- Kinase from .

Proteins associated to the PI3K/AKT/mTOR signaling pathway are widely used targets for cancer treatment, and in recent years they have also been evaluated as putative targets in trypanosomatids parasites, such as . Here, we performed a virtual screening approach to find candidates that can bind regions on or near the Pleckstrin homology domain of an AKT- protein in The compounds were also evaluated in vitro. The in silico and experimental results allowed us to identify a set of compounds that can potentially alter the intracellular signaling pathway through the AKT- kinase of the parasite; among them, a derivative of the pyrazolopyridine nucleus with an IC of 14.25 ± 1.00 μM against amastigotes of . In addition, we built a protein⁻protein interaction network of to understand the role of the AKT- protein in the parasite, and look for additional proteins that can be postulated as possible novel molecular targets for the rational design of compounds against .