School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China.
Molecules. 2018 Dec 11;23(12):3280. doi: 10.3390/molecules23123280.
We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress. RTCA is attracting more and more attention. Now it plays an important role in drug screening, toxicology, Chinese herbal medicine and so on. It has wide application prospects in the area of modern pharmaceutical evaluation and analysis.
为丰富和拓展 RTCA 技术在中医药领域的应用，我们对近年来 xCELLigence 实时细胞分析（RTCA）技术的应用进展进行了总结。RTCA 系统是一种已确立的电子细胞生物传感器。该系统采用微电极生物传感器技术，可实时、无标记、动态、非侵袭性地监测细胞活力、迁移、生长、扩散和增殖。我们从原理、特点、应用等方面，特别是从最新的应用进展方面，对 RTCA 技术的相关实验和文献进行了总结。RTCA 技术正受到越来越多的关注，目前在药物筛选、毒理学、中草药等方面发挥着重要作用，在现代药物评价分析领域具有广阔的应用前景。
