Holý Ondřej, Cruz-Córdova Ariadnna, Xicohtencatl-Cortes Juan, Hochel Igor, Parra-Flores Julio, Petrželová Jana, Fačevicová Kamila, Forsythe Stephen, Alsonosi Abdlrhman
Department of Public Health, Palacký University Olomouc, Olomouc, Czech Republic.
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico.
Microb Pathog. 2019 Feb;127:250-256. doi: 10.1016/j.micpath.2018.12.011. Epub 2018 Dec 11.
Cronobacter spp. are Gram-negative, facultative-anaerobic, non-spore forming, enteric coliform bacteria, which belongs to the Enterobacteriaceae family. Cronobacter spp. are opportunistic pathogens that have brought rare but life-threatening infections such as meningitis, necrotizing enterocolitis and bloodstream infections in neonates and infants. Information on the diversity, pathogenicity and virulence of Cronobacter species obtained from various sources is still relatively scarce and fragmentary. The aim of this study was to examine and analyse different pathogenicity and virulence factors among C. sakazakii and C. malonaticus strains isolated from clinical samples.
The thirty-six clinical Cronobacter strains have been used in this study. This bacterial collection consists of 25 strains of C. sakazakii and 11 strains of C. malonaticus, isolated from different clinical materials. Seven genes (ompA, inv, sip, aut, hly, fliC, cpa) were amplified by PCR. Moreover, the motility and the ability of these strains to adhere and invade human colorectal adenocarcinoma (HT-29) and mouse neuroblastoma (N1E-115) cell lines were investigated.
Our results showed that all tested strains were able to adhere to both used cell lines, HT-29 and N1E-115 cells. The invasion assay showed that 66.7% (24/36) of isolates were able to invade N1-E115 cells while 83% (30/36) of isolates were able to invade HT-29 cells. On the average, 68% of the C. sakazakii strains exhibited seven virulence factors and only 18% in C. malonaticus. All strains amplified ompA and fliC genes. The other genes were detected as follow: sip 97% (35/36), hlyA 92% (33/36), aut 94% (34/36), cpa 67% (24/36), and inv 69% (25/36).
C. sakazakii and C malonaticus strains demonstrate the diversity of the virulence factors present among these pathogens. It is necessary to permanently monitor the hospital environment to appropriately treat and resolve cases associated with disease. Furthermore, in-depth knowledge is needed about the source and transmission vehicles of pathogens in hospitals to adopt pertinent prevention measures.
阪崎肠杆菌属革兰氏阴性、兼性厌氧、无芽孢形成的肠道大肠埃希菌，属于肠杆菌科。阪崎肠杆菌属是机会致病菌，可在新生儿和婴儿中引发罕见但危及生命的感染，如脑膜炎、坏死性小肠结肠炎和血流感染。从各种来源获得的关于阪崎肠杆菌属物种的多样性、致病性和毒力的信息仍然相对稀少且零散。本研究的目的是检测和分析从临床样本中分离出的阪崎肠杆菌和丙二酸阪崎肠杆菌菌株之间不同的致病性和毒力因子。
本研究使用了36株临床阪崎肠杆菌菌株。该细菌集合包括从不同临床材料中分离出的25株阪崎肠杆菌和11株丙二酸阪崎肠杆菌。通过聚合酶链反应（PCR）扩增7个基因（ompA、inv、sip、aut、hly、fliC、cpa）。此外，还研究了这些菌株的运动性以及它们黏附并侵袭人结肠腺癌（HT-29）和小鼠神经母细胞瘤（N1E-115）细胞系的能力。
我们的结果表明，所有测试菌株都能够黏附到所用的细胞系HT-29和N1E-115细胞上。侵袭试验表明，66.7%（24/36）的分离株能够侵袭N1-E115细胞，而83%（30/36）的分离株能够侵袭HT-29细胞。平均而言，68%的阪崎肠杆菌菌株表现出7种毒力因子，而丙二酸阪崎肠杆菌中只有18%。所有菌株均扩增出ompA和fliC基因。其他基因的检测结果如下：sip为97%（35/36），hlyA为92%（33/36），aut为94%（34/36），cpa为67%（24/36），inv为69%（25/36）。
阪崎肠杆菌和丙二酸阪崎肠杆菌菌株显示出这些病原体中毒力因子的多样性。有必要持续监测医院环境，以妥善治疗和解决与疾病相关的病例。此外，需要深入了解医院中病原体的来源和传播媒介，以采取相关的预防措施。
