Shih M C, Heinrich P, Goodman H M
Department of Genetics, Harvard Medical School, Boston, MA.
Science. 1988 Nov 25;242(4882):1164-6. doi: 10.1126/science.3055302.
Nucleotide sequences for the nuclear genes encoding chloroplast (GapA and GapB) and cytosolic (GapC) glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) from Arabidopsis thaliana were determined. Comparison of nucleotide sequences indicates that the divergence of chloroplast and cytosolic GAPDH genes preceded the divergence of prokaryotes and eukaryotes. In addition, some intron-exon junctions are conserved among GapB, GapC, and chicken GAPDH genes. These results provide evidence at the molecular level to support the idea that introns existed before the divergence of prokaryotes and eukaryotes.
测定了拟南芥中编码叶绿体（GapA和GapB）和胞质（GapC）甘油醛-3-磷酸脱氢酶（GAPDHs）的核基因的核苷酸序列。核苷酸序列比较表明，叶绿体和胞质GAPDH基因的分化先于原核生物和真核生物的分化。此外，GapB、GapC和鸡GAPDH基因之间的一些内含子-外显子连接是保守的。这些结果在分子水平上提供了证据，支持内含子在原核生物和真核生物分化之前就已存在的观点。
