Chaubet Guilhem, Thoreau Fabien, Wagner Alain
University of Strasbourg, Faculty of Pharmacy, France.
University of Strasbourg, Faculty of Pharmacy, France.
Drug Discov Today Technol. 2018 Dec;30:21-26. doi: 10.1016/j.ddtec.2018.09.002. Epub 2018 Sep 27.
This review will discuss recent development in the bioconjugation of lysine residues on antibodies. As several chemoselective reagents have already been developed for modifying amine groups, recent strategies now tend to aim at being site-specific. Four general methods have been listed: kinetically controlled, template-directed or enzymatic strategies as well as the use of chemically programmed antibodies.
本综述将讨论抗体上赖氨酸残基生物共轭的最新进展。由于已经开发了几种用于修饰胺基的化学选择性试剂，目前的最新策略倾向于实现位点特异性。本文列出了四种一般方法：动力学控制、模板导向或酶促策略以及化学编程抗体的使用。
