Mura Maria Consuelo, Luridiana Sebastiano, Pulinas Luisa, Di Stefano Maria Veronica, Carcangiu Vincenzo
Department of Veterinary Medicine, Sassari University, Via Vienna 2, 07100, Sassari, Italy.
Department of Veterinary Medicine, Sassari University, Via Vienna 2, 07100, Sassari, Italy.
Anim Reprod Sci. 2019 Jan;200:67-74. doi: 10.1016/j.anireprosci.2018.11.012. Epub 2018 Nov 28.
The aims were to evaluate the reproductive response to ram placement with Sarda ewes with different allelic variants at position g.15099485 A > G of the MTNR1 A gene. Ram placements occurred between the early and late spring and there was analysis of whether this polymorphism is associated with other nucleotide substitutions. In each of the eight farms where the study was conducted (named F1-F8), 150 ewes (50 with A/A, A/G and G/G genotypes) were selected. In each group of 150 ewes, eight males were joined with ewes on the following dates: 25 March (T1) for F1-F2, 15 April (T2) for F3-F4, 5 May (T3) for F5-F6, and 1 June (T4) for F7-F8. The lambing dates and number of new born lambs were recorded until 220 days after joining rams with ewes. The ewes with G/G or A/G genotypes had a greater fertility rate compared with those with A/A in T1, T2 (P < 0.01), and in T3 and T4 (P < 0.05). The duration of time in days from the time of ram joining with ewes to lambing was less in the ewes with G/G and A/G compared with those with A/A (P < 0.01). The g.15099485A>G variation was always associated with that at position g.15099391G>A. Results indicate there is a positive effect of the g.15099485A>G variant on reproduction when males were joined with ewes in March or April. The association that was ascertained in this study between the g.15099391G>A and g.15099485A>G polymorphisms for the MTNR1 A gene, could provide new insights to clarify the mechanism of action of melatonin.
本研究旨在评估在MTNR1 A基因g.15099485 A > G位点具有不同等位基因变体的撒丁岛母羊对放入公羊后的繁殖反应。公羊在早春和晚春期间放入，同时分析该多态性是否与其他核苷酸替换相关。在开展本研究的八个农场（命名为F1 - F8）中，每个农场挑选了150只母羊（50只具有A/A、A/G和G/G基因型）。在每组150只母羊中，八只公羊在以下日期与母羊合群：3月25日（T1）用于F1 - F2农场，4月15日（T2）用于F3 - F4农场，5月5日（T3）用于F5 - F6农场，6月1日（T4）用于F7 - F8农场。记录母羊与公羊合群后直至220天的产羔日期和新生羔羊数量。在T1、T2（P < 0.01）以及T3和T4（P < 0.05）阶段，具有G/G或A/G基因型的母羊生育率高于具有A/A基因型的母羊。与具有A/A基因型的母羊相比，具有G/G和A/G基因型的母羊从与公羊合群到产羔的天数更短（P < 0.01）。g.15099485A>G变异始终与g.15099391G>A位点的变异相关。结果表明，当3月或4月将公羊与母羊合群时，g.15099485A>G变体对繁殖有积极影响。本研究确定的MTNR1 A基因g.15099391G>A和g.15099485A>G多态性之间的关联，可为阐明褪黑素的作用机制提供新的见解。
