血栓性血小板减少性紫癜中血清前列环素结合的定量分析。

Quantitation of serum prostacyclin-binding in thrombotic thrombocytopenic purpura.

作者信息

Tsai A L, Manner C E, Rudersdorf T, Wu K K

机构信息

Department of Internal Medicine, University of Texas Health Science Center, Houston 77225.

出版信息

Thromb Res. 1988 Sep 15;51(6):583-92. doi: 10.1016/0049-3848(88)90142-9.

DOI:10.1016/0049-3848(88)90142-9

PMID:3055418

Abstract

We quantitated serum PGI2 binding of 8 normal subjects and two TTP (thrombotic thrombocytopenic purpura) patients by gel filtration and gel partition methods using a stable PGI2 analogue, iloprost. The dissociation constant (KD) and the binding capacity (or binding stoichiometry) determined for the normals were 94 +/- S.D. 19 microM and 1.8 +/- S.D. 0.5 mM (or 2.0 +/- .6, iloprost:HSA). Corresponding values for serum samples obtained from TTP patient I were KD 200 microM, and Bmax 2.3 mM in the acute phase, and 75 microM and 1.8 mM respectively in the remission phase. The serum samples from TTP patient II exhibited a higher KD. Values of 299 microM (acute phase) and 147 microM (remission phase) were obtained. The corresponding binding capacities were 2.1 mM and 1.5 mM. Binding affinity change appears to be the main factor which resulted in the PGI2 binding defect in TTP.

摘要

我们使用稳定的前列环素类似物伊洛前列素，通过凝胶过滤和凝胶分配法对8名正常受试者和两名血栓性血小板减少性紫癜（TTP）患者的血清前列环素I2（PGI2）结合情况进行了定量分析。正常受试者的解离常数（KD）和结合能力（或结合化学计量）分别为94±标准差19微摩尔和1.8±标准差0.5毫摩尔（或2.0±0.6，伊洛前列素：人血清白蛋白）。从TTP患者I急性期获得的血清样本的相应值为KD 200微摩尔，Bmax 2.3毫摩尔，缓解期分别为75微摩尔和1.8毫摩尔。TTP患者II的血清样本表现出更高的KD，急性期值为299微摩尔，缓解期值为147微摩尔，相应的结合能力分别为2.1毫摩尔和1.5毫摩尔。结合亲和力变化似乎是导致TTP中PGI2结合缺陷的主要因素。