Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña Nº 352, (B1876BXD), Bernal, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas - CONICET, Godoy Cruz Nº 2290 (C1425FQB), Ciudad de Buenos Aires, Argentina.
World J Microbiol Biotechnol. 2018 Dec 15;35(1):5. doi: 10.1007/s11274-018-2577-6.
In the present study, we evaluated the transcriptional response of four stress-related genes in three Oenococcus oeni strains after acclimation at two different temperatures. Gene expression was analyzed at time zero and after 48 h acclimation at 18 and 21 °C. After the acclimation period cells were inoculated into sterile Pinot noir wine and MLF was followed for 25 days to investigate if different acclimation temperatures could influence cell survival and MLF performance. L-malic acid consumption, population survival, and transcriptional behavior were different upon the acclimation temperature. rmlB and hsp20 genes presented a considerable increase in their expression level when strains were acclimated at 18 °C particularly in the psychrotrophic strains UNQOe19 and UNQOe4 isolated from Patagonian Pinot noir wine in comparison with the control strain (ATCC 27310). The increase in rmlB and hsp20 expression could account for the better survival of these strains in Pinot noir in comparison with the control strain. In addition, Patagonian populations acclimated at 18 °C were able to consume a higher percentage of L-malic acid in comparison with cells acclimated at 21 °C. Our results suggest that gene expression analysis of cells acclimated at sub-optimal temperatures could benefit the selection of psychrotrophic strains aimed as starter cultures.
在本研究中，我们评估了在两种不同温度下适应后，三种酒香酵母菌株中 4 个与应激相关的基因的转录反应。在 0 小时和 48 小时适应 18 和 21°C 后分析基因表达。适应期结束后，将细胞接种到无菌黑比诺葡萄酒中，并进行 25 天的 MLF 以研究不同的适应温度是否会影响细胞存活和 MLF 性能。在适应温度下，L-苹果酸消耗、种群存活和转录行为存在差异。当菌株在 18°C 下适应时，rmlB 和 hsp20 基因的表达水平显著增加，与对照菌株（ATCC 27310）相比，特别是从巴塔哥尼亚黑比诺葡萄酒中分离的嗜冷菌株 UNQOe19 和 UNQOe4。rmlB 和 hsp20 表达的增加可以解释这些菌株在黑比诺葡萄酒中的更好存活，与对照菌株相比。此外，在 18°C 下适应的巴塔哥尼亚种群能够消耗更高比例的 L-苹果酸，与在 21°C 下适应的细胞相比。我们的研究结果表明，对适应亚最佳温度的细胞进行基因表达分析可能有助于选择作为起始培养物的嗜冷菌株。
