富硒酵母改善黄曲霉毒素 B1 诱导的小鼠生长性能、抗氧化能力、IL-2 和 IFN-γ 含量及基因表达变化的影响。
Effects of Selenium-Enriched Yeast Improved Aflatoxin B1-Induced Changes in Growth Performance, Antioxidation Capacity, IL-2 and IFN-γ Contents, and Gene Expression in Mice.
机构信息
College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
出版信息
Biol Trace Elem Res. 2019 Sep;191(1):183-188. doi: 10.1007/s12011-018-1607-4. Epub 2018 Dec 15.
Sixty Kunming mice were randomly assigned into three groups. Mice in a control group were fed a basal diet, while mice in AFB1 group and AFB1-Se group were fed the basal diet supplemented with 250 μg/kg AFB1 or the basal diet supplemented with 250 μg/kg AFB1 and 0.2 mg/kg selenium as selenium-enriched yeast, respectively. On day 30 of the experiment, growth performance, glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) levels, and malondialdehyde (MDA) contents in liver, interleukin-2 (IL-2), and interferon-γ (IFN-γ) contents in serum, and cytochrome P3a11 (Cyp3a11), IL-2, IFN-γ, and GSH-Px1 mRNA levels in liver were determined. The results showed that final weights, weight gains, T-AOC levels, GSH-Px1, and IFN-γ mRNA levels in AFB1-Se group and control group were higher or significantly higher than those in AFB1 group (P < 0.05 or P < 0.01), respectively. Body length gains in AFB1 group were lower than those in the control group (P < 0.05), while there was no significant difference between the AFB1-Se and control groups (P > 0.05). IL-2 contents and liver IL-2 mRNA levels in AFB1-Se group were significantly higher than those in the AFB1 group and control group (P < 0.01), and IL-2 contents in the control group were also significantly higher than those in the AFB1 group (P < 0.01). IFN-γ contents in AFB1-Se group and AFB1 group were significantly higher than those in control group (P < 0.01), while IFN-γ contents in AFB1-Se group were significantly lower than those in AFB1 group (P < 0.01). Cyp3a11 mRNA levels in AFB1-Se group and AFB1 group were significantly higher than those in the control group (P < 0.01). The results indicated that selenium-enriched yeast could partly reduce the toxicity induced by AFB1 in mice, including improving growth performance, antioxidation capacity, IL-2 and IFN-γ contents, and enhancing IL-2, IFN-γ, and GSH-Px1 mRNA levels.
60 只昆明小鼠被随机分为三组。对照组小鼠喂食基础日粮，而 AFB1 组和 AFB1-Se 组小鼠分别喂食基础日粮，补充 250μg/kg AFB1 或基础日粮，补充 250μg/kg AFB1 和 0.2mg/kg 硒作为富硒酵母。实验第 30 天，测定生长性能、谷胱甘肽过氧化物酶（GSH-Px）活性、总抗氧化能力（T-AOC）水平和肝组织丙二醛（MDA）含量、血清白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）含量以及肝组织细胞色素 P3a11（Cyp3a11）、IL-2、IFN-γ 和 GSH-Px1mRNA 水平。结果表明，AFB1-Se 组和对照组的终重、增重、T-AOC 水平、GSH-Px1 和 IFN-γmRNA 水平均高于或显著高于 AFB1 组（P<0.05 或 P<0.01）。AFB1 组的体长增加量低于对照组（P<0.05），而 AFB1-Se 组与对照组之间无显著差异（P>0.05）。AFB1-Se 组的 IL-2 含量和肝组织中 IL-2mRNA 水平均显著高于 AFB1 组和对照组（P<0.01），对照组的 IL-2 含量也显著高于 AFB1 组（P<0.01）。AFB1-Se 组和 AFB1 组的 IFN-γ 含量均显著高于对照组（P<0.01），而 AFB1-Se 组的 IFN-γ 含量显著低于 AFB1 组（P<0.01）。AFB1-Se 组和 AFB1 组的 Cyp3a11mRNA 水平均显著高于对照组（P<0.01）。结果表明，富硒酵母可部分减轻 AFB1 对小鼠的毒性，包括提高生长性能、抗氧化能力、IL-2 和 IFN-γ 含量，以及增强 IL-2、IFN-γ 和 GSH-Px1mRNA 水平。
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