Taunk Jyoti, Rani Asha, Yadav Neelam R, Vartyadav Dev, Yadav Ram C, Raj Kushal, Kumar Ramesh, Yadav H P
Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 004, India.
J Genet. 2018 Dec;97(5):1241-1251.
Downy mildew (DM) caused by is the most calamitous disease of pearl millet. Therefore, for introgression of DM resistance (DMR) in HHB 197 (MH-1302), an elite pearl millet hybrid, a marker-assisted breeding was undertaken by targeting three DMR loci on linkage groups (LGs) 1, 2 and 4. Breeding programme was initiated by crossing HBL 11 (DM susceptible), male parent of HHB 197 hybrid with ICMP 451 (DM-resistant) to produce true F plants. By conducting three rounds of backcrossing and selection, BCF lines were generated. Foreground selection was employed using six polymorphic simple sequence repeat (SSR) markers of the 18 total selected markers. Four of these markers were linked to LG 1, five to LG 2 and nine to LG 4. Background selection was performed in BCF generation using 33 polymorphic SSR markers of a total of 56 evenly spread SSR markers in the pearl millet genome to check recovery of recurrent parent genome. On the basis of genotypic selection (foreground as well as background) using selected SSR markers, agronomic performance in field and DM screening in greenhouse; 10 improved HBL 11 lines were selected and crossed with ICMA 97111 to produce DM-resistant HHB 197 hybrid versions. Six putatively improved HHB 197 hybrids were successfully tested in first year trials at Hisar and Bawal locations of Haryana and two selected versions with higher yield and zero DM incidence will be further tested in multilocation trials.
由[病原菌名称未给出]引起的霜霉病（DM）是珍珠粟最具灾难性的病害。因此，为了将霜霉病抗性（DMR）导入优良珍珠粟杂交种HHB 197（MH - 1302），通过针对连锁群（LG）1、2和4上的三个DMR基因座进行了标记辅助育种。育种计划始于将HHB 197杂交种的父本易感霜霉病的HBL 11与抗霜霉病的ICMP 451杂交，以产生真F植株。通过进行三轮回交和选择，获得了BCF株系。在总共选择的18个标记中，使用6个多态性简单序列重复（SSR）标记进行前景选择。其中4个标记与LG 1连锁，5个与LG 2连锁, 9个与LG 4连锁。在BCF世代中，使用珍珠粟基因组中总共56个均匀分布的SSR标记中的33个多态性SSR标记进行背景选择，以检查轮回亲本基因组的恢复情况。基于使用选定SSR标记的基因型选择（前景和背景）、田间农艺性能和温室霜霉病筛选；选择了10个改良的HBL 11株系，并与ICMA 97111杂交，以产生抗霜霉病的HHB 197杂交种版本。6个推定改良的HHB 197杂交种在哈里亚纳邦希萨尔和巴瓦尔地点的第一年试验中成功进行了测试，两个选定的高产且霜霉病发病率为零的版本将在多点试验中进一步测试。
