Stein Liana Roberts, Zorumski Charles F, Izumi Yukitoshi
Department of Psychiatry, Washington University School of Medicine, Campus Box 8134, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
The Taylor Family Institute for Innovative Psychiatric Research, Washington University School of Medicine, Campus Box 8134, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Oruen. 2017 Dec;3(2):94-101.
The rodent hippocampal slice preparation has long been a critical tool for studying the electrophysiological effects of pharmacological and genetic manipulations. Slices can be prepared with several different slicing methods including the tissue chopper, vibratome, and rotary slicer. To examine how slicing methods affect slice integrity, we generated hippocampal slices by these three methods and compared their histology and electrophysiological responses. Although all three methods generate histological alterations, the time course is slowest in slices generated with a rotary slicer. Furthermore, although paired-pulse facilitation in dendritic field EPSPs was observed in slices generated by all three methods, paired-pulse potentiation in population spikes, which is common in chopper- and vibratome-generated slices was seldom observed in rotary-generated slices, suggesting less disinhibiton. For preservation of hippocampal slice integrity, the rotary slicer may offer advantages over the other two devices.
长期以来，啮齿动物海马切片制备一直是研究药理学和基因操作的电生理效应的关键工具。可以使用几种不同的切片方法制备切片，包括组织切片机、振动切片机和旋转切片机。为了研究切片方法如何影响切片完整性，我们通过这三种方法生成海马切片，并比较它们的组织学和电生理反应。尽管所有三种方法都会产生组织学改变，但旋转切片机生成的切片中变化的时间进程最慢。此外，尽管在所有三种方法生成的切片中均观察到树突场兴奋性突触后电位（EPSP）中的双脉冲易化，但群体峰电位中的双脉冲增强在旋转切片机生成的切片中很少观察到，而在切片机和振动切片机生成的切片中很常见，这表明抑制解除较少。为了保存海马切片的完整性，旋转切片机可能比其他两种设备更具优势。
