Molecular Neuroimaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Neurosci Res. 2012 Mar;72(3):275-8. doi: 10.1016/j.neures.2011.11.001. Epub 2011 Nov 12.
We described the preparation of acute living slices from the hippocampus of the neonatal common marmoset (Callithrix jacchus). Slices from a temporal lobe section were prepared quickly using a rotary slicer. By this method, we successfully recorded field potentials, namely, pre-synaptic fiber volley and field excitatory post-synaptic potentials, from the hippocampal CA1 region with conventional electrophysiological techniques, and analyzed the indicators of synaptic function such as input-output curve. This study thus presents an efficient preparation method for acute living hippocampal slice from which synaptic function of the hippocampus in non-human primate can be analyzed.
我们描述了从新生普通狨猴(Callithrix jacchus)海马体中制备急性活体切片的方法。使用旋转切片机快速制备颞叶切片。通过这种方法,我们成功地使用常规电生理技术从海马 CA1 区记录了场电位,即突触前纤维放电和场兴奋性突触后电位,并分析了突触功能的指标,如输入-输出曲线。因此,本研究提出了一种从非人类灵长类动物海马体中分析突触功能的急性活体海马切片的有效制备方法。