Department of Urology, Affiliated Hospital of Guilin Medical University, Guilin, China.
Eur Rev Med Pharmacol Sci. 2018 Dec;22(23):8151-8160. doi: 10.26355/eurrev_201812_16507.
Bladder cancer is the most prevalent genitourinary malignant disorder worldwide. We aimed to observe effects of high-glucose on bladder cancer proliferation and explore the associated mechanisms.
Human bladder cancer cell line, T24, was divided into Blank, Control (Ctrl), 10 mmol/l, 20 mmol/l and 30 mmol/l group. T24 cell proliferation was evaluated by using multiple table tournament (MTT) assay and colony formation analysis, respectively. Quantitative Real-time PCR (qRT-PCR) assay was employed to examine mRNA expression of Wnt-5a and β-catenin. Meanwhile, Western blot assay was used to evaluate expression of Wnt-5a and β-catenin protein. The linear regression analysis was utilized to analyze correlation between Wnt-5a/β-catenin expression and T24 cell proliferation.
High-glucose significantly enhanced proliferation of T24 cells compared to that of Blank and Ctrl group (p < 0.05). High-glucose significantly promoted colony formation of T24 cells compared to that of Blank and Ctrl group (p < 0.05). High-glucose significantly up-regulated Wnt-5a mRNA and protein expression compared to that of Blank and Ctrl group (p < 0.01). High-glucose significantly increased β-catenin mRNA and protein expression compared to that of Blank and Ctrl group (p < 0.01). Effects of high-glucose on T24 cell proliferation were increased following with the enhanced glucose concentration. Wnt/β-catenin signaling pathway molecules were correlated with colony formation of T24 cells (p < 0.05).
High-glucose promoted the proliferation of T24 cells by activating the Wnt/β-catenin signaling pathway. This study would provide the novel targets for bladder cancer therapy.
膀胱癌是全球最常见的泌尿生殖系统恶性疾病。本研究旨在观察高糖对膀胱癌增殖的影响，并探讨其相关机制。
将人膀胱癌细胞系 T24 分为空白组、对照组（Ctrl）、10mmol/L、20mmol/L 和 30mmol/L 组。分别采用多组淘汰赛（MTT）检测和集落形成实验检测 T24 细胞增殖情况。采用实时定量 PCR（qRT-PCR）检测 Wnt-5a 和 β-连环蛋白的 mRNA 表达。同时，采用 Western blot 检测 Wnt-5a 和 β-连环蛋白蛋白的表达。采用线性回归分析 Wnt-5a/β-连环蛋白表达与 T24 细胞增殖的相关性。
与空白组和对照组相比，高糖组明显增强了 T24 细胞的增殖（p＜0.05）。与空白组和对照组相比，高糖组明显促进了 T24 细胞的集落形成（p＜0.05）。与空白组和对照组相比，高糖组明显上调了 Wnt-5a 的 mRNA 和蛋白表达（p＜0.01）。与空白组和对照组相比，高糖组明显增加了β-连环蛋白的 mRNA 和蛋白表达（p＜0.01）。随着葡萄糖浓度的增加，高糖对 T24 细胞增殖的影响也随之增加。Wnt/β-连环蛋白信号通路分子与 T24 细胞的集落形成相关（p＜0.05）。
高糖通过激活 Wnt/β-连环蛋白信号通路促进了 T24 细胞的增殖。本研究为膀胱癌的治疗提供了新的靶点。
