Department of Stomatology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China.
Eur Rev Med Pharmacol Sci. 2018 Dec;22(23):8315-8323. doi: 10.26355/eurrev_201812_16529.
The aim of this study was to explore whether maternally expressed gene 3 (MEG3) could facilitate the proliferation and migration of oral squamous cell carcinoma (OSCC) cells by selectively binding to miR-21, thereby participating in the progression of OSCC.
The expression levels of MEG3 and miR-21 in OSCC tissues and normal control tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The effects of MEG3 and miR-21 on cell proliferation and migration were examined by cell counting kit-8 (CCK-8), transwell, and scratch assay, respectively. Meanwhile, cell cycle was detected using flow cytometry. The binding relationship between miR-21 and MEG3 was confirmed by dual luciferase assay. In addition, MEG3 and miR-21 were simultaneously knock-down to figure out whether MEG3 could regulate the proliferation and migration of OSCC cells through targeted binding to miR-21.
QRT-PCR results indicated that MEG3 expression in OSCC tissues was remarkably lower than that of normal control tissues. However, the expression of miR-21 was significantly higher in OSCC tissues. Meanwhile, it was found that inhibiting MEG3 expression in OSCC cell lines could significantly promote cell proliferation and migration, while the simultaneous inhibition of miR-21 showed the opposite effect. Dual Luciferase assay results revealed that MEG3 could selectively bind to miR-21. In addition, we demonstrated that the knockdown of MEG3 in Tca-8113 and CAL-27 cells partially reversed the inhibitory effect of downregulated-miR-21 on cell proliferation and migration. These results further suggested that MEG3 might regulate OSCC cell proliferation via selectively binding to miR-21.
Low expression of MEG3 can promote the proliferation and migration of OSCC cells through targeted binding to miR-21.
本研究旨在探讨母系表达基因 3（MEG3）是否可以通过选择性结合 miR-21 促进口腔鳞状细胞癌（OSCC）细胞的增殖和迁移，从而参与 OSCC 的进展。
采用实时定量聚合酶链反应（qRT-PCR）检测 OSCC 组织和正常对照组织中 MEG3 和 miR-21 的表达水平。通过细胞计数试剂盒-8（CCK-8）、Transwell 和划痕实验分别检测 MEG3 和 miR-21 对细胞增殖和迁移的影响。同时，采用流式细胞术检测细胞周期。通过双荧光素酶报告基因实验证实 miR-21 与 MEG3 的结合关系。此外，同时敲低 MEG3 和 miR-21，以明确 MEG3 是否可以通过靶向结合 miR-21 调节 OSCC 细胞的增殖和迁移。
qRT-PCR 结果表明，OSCC 组织中 MEG3 的表达明显低于正常对照组织。然而，OSCC 组织中 miR-21 的表达显著升高。同时，发现抑制 OSCC 细胞系中 MEG3 的表达可显著促进细胞增殖和迁移，而同时抑制 miR-21 则表现出相反的效果。双荧光素酶报告基因实验结果显示，MEG3 可以选择性地结合 miR-21。此外，我们证明在 Tca-8113 和 CAL-27 细胞中敲低 MEG3 可以部分逆转下调 miR-21 对细胞增殖和迁移的抑制作用。这些结果进一步表明，MEG3 可能通过选择性结合 miR-21 调节 OSCC 细胞的增殖。
低表达的 MEG3 可以通过靶向结合 miR-21 促进 OSCC 细胞的增殖和迁移。
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