Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Department of Chemical Engineering, National Institute of Technology, Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.
Colloids Surf B Biointerfaces. 2019 Mar 1;175:403-408. doi: 10.1016/j.colsurfb.2018.12.008. Epub 2018 Dec 5.
The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400-440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.
叶绿素 a（Chla）的 J-聚集体在天然系统中充当光捕获天线。在这项研究中，我们采用了由长链 1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱（DMPC）和短链 1,2-二己酰基-sn-甘油-3-磷酸胆碱（DHPC）组成的磷脂膜作为诱导 Chla 聚集体的平台。在总脂质浓度为 20 mM 的条件下，在混合比（q）= 1.5 时，DMPC/DHPC 组装体诱导了 Chla J-聚集体在 20°C 下形成。然而，在 q = ∞（DMPC 囊泡）和 q = 0（DHPC 胶束）时，膜中未观察到 Chla 聚集体。根据 Soret 带光谱（400-440nm）的解卷积分析，估计 DMPC/DHPC 中 q = 1.5 处的周围 Chla 分子包含一个极性较小的环境。在较低的脂质浓度条件下研究了 Chla J-聚集体的光还原活性。
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