Liu Chun Xia, Geng Li Zhao, Xu Jian Ping
Syngenta Beijing Innovation Center, Beijing 102206, China.
Yi Chuan. 2018 Dec 20;40(12):1075-1091. doi: 10.16288/j.yczz.18-079.
The life science has entered a new chapter with the revolutionary implementation of the CRISPR/Cas9 genome editing technology in various living organisms. With the unique flexibility, feasibility and extendibility, the CRISPR/Cas9 technology greatly accelerates genetic engineering research, as well as plant molecular breeding. However, it has become a challenge to screen for and identify genome-edited plants at early stages in a rapid and high-throughput fashion, due to the massive number of plants produced from transformation process. In this review, we summarize the molecular methods developed in recent years to identify genome-edited plants. We compare their advantages and disadvantages, and the scope of application. In addition, we provide insights of the development trend of detection methods for plant genome editing. This review will serve as a reference for future genome editing research in plants.
随着CRISPR/Cas9基因组编辑技术在各种生物体中的革命性应用,生命科学进入了一个新篇章。凭借其独特的灵活性、可行性和可扩展性,CRISPR/Cas9技术极大地加速了基因工程研究以及植物分子育种。然而,由于转化过程产生的植物数量众多,如何快速、高通量地在早期筛选和鉴定基因组编辑植物已成为一项挑战。在本综述中,我们总结了近年来开发的用于鉴定基因组编辑植物的分子方法。我们比较了它们的优缺点和应用范围。此外,我们还对植物基因组编辑检测方法的发展趋势提供了见解。本综述将为未来植物基因组编辑研究提供参考。