Engineering Physics Department, Ecole Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Montréal, Québec H3C 3A7, Canada.
Analyst. 2019 Feb 21;144(4):1303-1308. doi: 10.1039/c8an01891j. Epub 2018 Dec 18.
We present the development of an innovative technology for quantitative multiplexed cytology analysis based on the application of spectrally distinctive plasmonic nanoparticles (NPs) as optical probes and on cost-effective side-illumination multispectral darkfield microscopy (SIM) as the differential NP imaging method. SIM is based on lateral illumination by arrays of discrete color RGB light emitting diodes (LEDs) of spectrally adjusted plasmonic NPs and consecutive detection by the conventional CMOS color camera. We demonstrate the enhanced contrast and higher resolution of our method for individual NP detection in the liquid medium and of NP markers attached on the cell membrane in a cytology preparation by comparing it to the conventional darkfield microscopy (DFM). The proposed illumination and detection system is compatible with current clinical microscopy equipment used by pathologists and can greatly simplify the adaptation of plasmonic NPs as novel reliable and stable biological multiplexed chromatic markers for biodetection and diagnosis.
我们提出了一种基于光谱独特等离子体纳米粒子（NPs）作为光学探针的创新定量多重细胞学分析技术的开发，以及作为差分 NP 成像方法的具有成本效益的侧向照明多光谱暗场显微镜（SIM）。SIM 基于离散 RGB 发光二极管（LED）阵列的侧向照明，这些 LED 的光谱经过调整以适用于等离子体 NPs，并且通过传统的 CMOS 彩色相机进行连续检测。我们通过将其与传统暗场显微镜（DFM）进行比较，证明了我们在液体介质中单个 NP 检测和细胞病理学制备中细胞膜上 NP 标记物的方法的增强对比度和更高分辨率。所提出的照明和检测系统与病理学家使用的当前临床显微镜设备兼容，并可以大大简化将等离子体 NPs 作为新型可靠和稳定的生物多重彩色标记物用于生物检测和诊断的适应过程。
