Leong M M, Fox G R
Department of Biology, University of Victoria, British Columbia, Canada.
Anal Biochem. 1988 Jul;172(1):145-50. doi: 10.1016/0003-2697(88)90423-x.
The application of 4-iodophenol as a signal enhancer for luminol-based immunodot and Western blotting assays was investigated. Immunodetection of biotin-bovine serum albumin (BSA) using a rat anti-biotin antibody for immunodot binding and goat anti-biotin peroxidase conjugate for Western blotting assays was employed to demonstrate the signal enhancement attainable with 4-iodophenol. The addition of 4-iodophenol at a final working concentration of 52.2 microM to a light-emitting substrate, luminol, resulted in 3-fold and 10-fold signal enhancements for Western blotting and immunodot binding detection of biotin-BSA, respectively.
研究了4-碘苯酚作为基于鲁米诺的免疫斑点法和蛋白质印迹分析信号增强剂的应用。采用大鼠抗生物素蛋白抗体进行免疫斑点结合，山羊抗生物素蛋白过氧化物酶偶联物进行蛋白质印迹分析，对生物素化牛血清白蛋白（BSA）进行免疫检测，以证明4-碘苯酚可实现的信号增强。在发光底物鲁米诺中加入终浓度为52.2微摩尔的4-碘苯酚，分别使生物素化BSA的蛋白质印迹检测和免疫斑点结合检测的信号增强3倍和10倍。
