Health Science Special Unit, Federal University of Goias, Jataí, Goiás, Brazil.
Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
J Texture Stud. 2019 Feb;50(1):45-52. doi: 10.1111/jtxs.12384. Epub 2019 Jan 17.
The aim of this study was to assess the differences in salivary biomarker levels of young adults classified according to body fat accumulation. One hundred and thirty-four volunteers were evaluated (mean age 21 ± 2 years). Body composition was calculated through skinfold thickness: supra-iliac, biceps, and triceps, sub-scapular. Body fat percentage (BF%) was used to classify subjects according to fat tissue accumulation: normal-weight (n = 37, 19 females, 18 males), overweight (n = 42, 30 females, 12 males), and obese (n = 55, 42 females, 13 males). Saliva samples were collected 30 min after awakening to determine salivary levels of 17-beta-estradiol. For salivary cortisol and alpha-amylase activity (sAA) three samples were obtained, just after awakening, 30 min after awakening and at bedtime. Oral contraceptive intake was considered for the female group. The results showed that overweight and obese females using oral contraceptive presented lower levels of 17-β-estradiol than normal-weight females. In overweight and obese males, sAA levels were higher 30 min post-awakening when compared with the normal-weight group. The comparison of sAA levels within time showed no difference for males; obese females showed significant higher values at bedtime than 30 min post-awakening. The salivary cortisol concentration showed higher values at morning decreasing significantly at bedtime for all groups. Concluding, differences in 17-β-estradiol and sAA levels were found in females and males, respectively, according to body fat accumulation, showing the usefulness of salivary biomarkers in the study of systemic conditions. PRACTICAL APPLICATIONS: Saliva is an advantageous biological fluid in innovative methods for diagnosis. Besides being a noninvasive method, salivary steroid measurements have the potential to provide a convenient assessment of serum free steroid concentrations.
这项研究的目的是评估根据体脂积累分类的年轻成年人的唾液生物标志物水平差异。评估了 134 名志愿者（平均年龄 21±2 岁）。通过皮褶厚度计算身体成分：髂上、二头肌和三头肌、肩胛下。体脂肪百分比（BF%）用于根据脂肪组织积累对受试者进行分类：正常体重（n=37，19 名女性，18 名男性）、超重（n=42，30 名女性，12 名男性）和肥胖（n=55，42 名女性，13 名男性）。在觉醒后 30 分钟收集唾液样本以确定 17-β-雌二醇的唾液水平。为了测定唾液皮质醇和α-淀粉酶活性（sAA），共采集了三个样本，分别在觉醒后、觉醒后 30 分钟和睡前。对于女性组，考虑了口服避孕药的摄入。结果表明，服用口服避孕药的超重和肥胖女性的 17-β-雌二醇水平低于正常体重女性。在超重和肥胖男性中，sAA 水平在觉醒后 30 分钟时高于正常体重组。在男性中，sAA 水平在时间内的比较没有差异；肥胖女性在睡前的 sAA 水平明显高于觉醒后 30 分钟。唾液皮质醇浓度在早晨较高，在所有组中均在睡前显著降低。结论，根据体脂积累，女性和男性的 17-β-雌二醇和 sAA 水平存在差异，表明唾液生物标志物在系统状况研究中的有用性。实际应用：唾液是一种具有创新性的诊断生物液体。除了非侵入性方法外，唾液类固醇测量还有可能提供对血清游离类固醇浓度的方便评估。
